Structural and functional insights into the bona fide catalytic state of Streptococcus pyogenes Cas9 HNH nuclease domain

Structural and functional insights into the bona fide catalytic state of Streptococcus pyogenes Cas9 HNH nuclease domain

The CRISPR-associated endonuclease Cas9 from Streptococcus pyogenes (SpyCas9), along with a programmable single-guide RNA (sgRNA), has been exploited as a significant genome-editing tool. Despite the recent advances in determining the SpyCas9 structures and DNA cleavage mechanism, the cleavage-compe...

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Bibliographic Details
Main Authors: Zhicheng Zuo, Ashwini Zolekar, Kesavan Babu, Victor JT Lin, Hamed S Hayatshahi, Rakhi Rajan, Yu-Chieh Wang, Jin Liu
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2019-07-01
Series:eLife
Subjects:
HEK293T cell
CRISPR-Cas9
catalysis model
Online Access:https://elifesciences.org/articles/46500

Internet

https://elifesciences.org/articles/46500

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