A Dynamic, Split-Luciferase-Based Mini-G Protein Sensor to Functionally Characterize Ligands at All Four Histamine Receptor Subtypes

A Dynamic, Split-Luciferase-Based Mini-G Protein Sensor to Functionally Characterize Ligands at All Four Histamine Receptor Subtypes

In drug discovery, assays with proximal readout are of great importance to study target-specific effects of potential drug candidates. In the field of G protein-coupled receptors (GPCRs), the determination of GPCR-G protein interactions and G protein activation by means of radiolabeled GTP analogs (...

Full description

Bibliographic Details
Main Authors: Carina Höring, Ulla Seibel, Katharina Tropmann, Lukas Grätz, Denise Mönnich, Sebastian Pitzl, Günther Bernhardt, Steffen Pockes, Andrea Strasser
Format: Article
Language:English
Published: MDPI AG 2020-11-01
Series:International Journal of Molecular Sciences
Subjects:
histamine receptors
split-luciferase complementation (SLC)
mini-G protein recruitment
G protein-coupled receptors (GPCRs)
histamine receptor ligands
bioluminescence
Online Access:https://www.mdpi.com/1422-0067/21/22/8440

Internet

https://www.mdpi.com/1422-0067/21/22/8440

Similar Items