Glucose-Limited Fed-Batch Cultivation Strategy to Mimic Large-Scale Effects in <i>Escherichia coli</i> Linked to Accumulation of Non-Canonical Branched-Chain Amino Acids by Combination of Pyruvate Pulses and Dissolved Oxygen Limitation
Insufficient mixing in large-scale bioreactors provokes gradient zones of substrate, dissolved oxygen (DO), pH, and other parameters. <i>E. coli</i> responds to a high glucose, low oxygen feeding zone with the accumulation of mixed acid fermentation products, especially formate, but also...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
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| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/9/6/1110 |