In vitro tripanocidal effect of 1,8-dioxooctahydroxanthenes (xanthenodiones) and tetraketones and improvement of cardiac parameters in vivo
Objective: Trypanosoma cruzi infection affects millions of people worldwide, and the drugs available for its treatment have limited efficacy. 1,8-Dioxooctahydroxanthenes and tetraketones are compounds with important biological applications. The aim of this study was to assess the trypanocidal and in...
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2020-09-01
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doaj-b19de0b4b0c3454da3e776b0e15439ec2021-05-20T07:49:20ZengElsevierJournal of Global Antimicrobial Resistance2213-71652020-09-0122466476In vitro tripanocidal effect of 1,8-dioxooctahydroxanthenes (xanthenodiones) and tetraketones and improvement of cardiac parameters in vivoAna Paula de Jesus Menezes0Milene Lopes da Silva1Wagner Luiz Pereira2Guilherme de Paula Costa3Aline Luciano Horta4Andréa Aparecida Santos Mendonça5Ana Claudia Alvarenga Carneiro6Debora Maria Soares de Souza7Rômulo Dias Novaes8Róbson Ricardo Teixeira9André Talvani10Immunobiology Laboratory of Inflammation, Department of Biological Sciences/ICEB, Federal University of Ouro Preto, Ouro Preto, MG, BrazilChemistry Department, Federal University of Viçosa, Viçosa, MG, BrazilChemistry Department, Federal University of Viçosa, Viçosa, MG, BrazilImmunobiology Laboratory of Inflammation, Department of Biological Sciences/ICEB, Federal University of Ouro Preto, Ouro Preto, MG, BrazilImmunobiology Laboratory of Inflammation, Department of Biological Sciences/ICEB, Federal University of Ouro Preto, Ouro Preto, MG, BrazilDepartment of Structural Biology, Federal University of Alfenas, Alfenas, MG, BrazilImmunobiology Laboratory of Inflammation, Department of Biological Sciences/ICEB, Federal University of Ouro Preto, Ouro Preto, MG, BrazilImmunobiology Laboratory of Inflammation, Department of Biological Sciences/ICEB, Federal University of Ouro Preto, Ouro Preto, MG, BrazilDepartment of Structural Biology, Federal University of Alfenas, Alfenas, MG, BrazilChemistry Department, Federal University of Viçosa, Viçosa, MG, Brazil; Corresponding author at: Laboratory of Immunobiology of Inflammation, DECBI/ICEB, Campus Morro do Cruzeiro, Universidade Federal de Ouro Preto, 35400-000 Ouro Preto, MG, Brazil.Immunobiology Laboratory of Inflammation, Department of Biological Sciences/ICEB, Federal University of Ouro Preto, Ouro Preto, MG, Brazil; Corresponding author at: Departamento de Química, Universidade Federal de Viçosa, 36570-900 Viçosa, MG, Brazil.Objective: Trypanosoma cruzi infection affects millions of people worldwide, and the drugs available for its treatment have limited efficacy. 1,8-Dioxooctahydroxanthenes and tetraketones are compounds with important biological applications. The aim of this study was to assess the trypanocidal and inflammatory activities of nine 1,8-dioxooctahydroxanthenes (1–9) and three tetraketones (10–12). Methods and results: By in vitro killing assay, three compounds were able to eliminate CL TdTomato expressing strain of T. cruzi, 9 (IC50 = 30.65 μM), 10 (IC50 = 14.11 μM), and 11 (IC50 = 26.43 μM). However, only 9 was not toxic to Vero cells. Next, to evaluate the in vivo antitrypanosomal and immunological efficacy of 9, Swiss mice were infected with the Y and CL strains of T. cruzi and treated for 10 days with 50 mg/kg of 9. This compound reduced the cardiac inflammatory infiltration in animals infected with both strains. Rank's ligand (RankL), CCL2, and interferon (IFN)-γ were measured in the cardiac tissue homogenate of the Y-strain-infected animals, and no interference of 9 was observed. However, compound 9 increased the RankL and interleukin (IL)-10 levels in CL-infected mice. No hepatic and renal toxicity was observed. Conclusion: Our findings showed that 1,8-dioxooctahydroxanthene has antiparasitic effect and ameliorates the cardiac inflammatory parameters related to T. cruzi infection.http://www.sciencedirect.com/science/article/pii/S2213716520300977Trypanosoma cruziXanthenodionesTetraketonesInflammationHeart damageBenznidazole |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ana Paula de Jesus Menezes Milene Lopes da Silva Wagner Luiz Pereira Guilherme de Paula Costa Aline Luciano Horta Andréa Aparecida Santos Mendonça Ana Claudia Alvarenga Carneiro Debora Maria Soares de Souza Rômulo Dias Novaes Róbson Ricardo Teixeira André Talvani |
spellingShingle |
Ana Paula de Jesus Menezes Milene Lopes da Silva Wagner Luiz Pereira Guilherme de Paula Costa Aline Luciano Horta Andréa Aparecida Santos Mendonça Ana Claudia Alvarenga Carneiro Debora Maria Soares de Souza Rômulo Dias Novaes Róbson Ricardo Teixeira André Talvani In vitro tripanocidal effect of 1,8-dioxooctahydroxanthenes (xanthenodiones) and tetraketones and improvement of cardiac parameters in vivo Journal of Global Antimicrobial Resistance Trypanosoma cruzi Xanthenodiones Tetraketones Inflammation Heart damage Benznidazole |
author_facet |
Ana Paula de Jesus Menezes Milene Lopes da Silva Wagner Luiz Pereira Guilherme de Paula Costa Aline Luciano Horta Andréa Aparecida Santos Mendonça Ana Claudia Alvarenga Carneiro Debora Maria Soares de Souza Rômulo Dias Novaes Róbson Ricardo Teixeira André Talvani |
author_sort |
Ana Paula de Jesus Menezes |
title |
In vitro tripanocidal effect of 1,8-dioxooctahydroxanthenes (xanthenodiones) and tetraketones and improvement of cardiac parameters in vivo |
title_short |
In vitro tripanocidal effect of 1,8-dioxooctahydroxanthenes (xanthenodiones) and tetraketones and improvement of cardiac parameters in vivo |
title_full |
In vitro tripanocidal effect of 1,8-dioxooctahydroxanthenes (xanthenodiones) and tetraketones and improvement of cardiac parameters in vivo |
title_fullStr |
In vitro tripanocidal effect of 1,8-dioxooctahydroxanthenes (xanthenodiones) and tetraketones and improvement of cardiac parameters in vivo |
title_full_unstemmed |
In vitro tripanocidal effect of 1,8-dioxooctahydroxanthenes (xanthenodiones) and tetraketones and improvement of cardiac parameters in vivo |
title_sort |
in vitro tripanocidal effect of 1,8-dioxooctahydroxanthenes (xanthenodiones) and tetraketones and improvement of cardiac parameters in vivo |
publisher |
Elsevier |
series |
Journal of Global Antimicrobial Resistance |
issn |
2213-7165 |
publishDate |
2020-09-01 |
description |
Objective: Trypanosoma cruzi infection affects millions of people worldwide, and the drugs available for its treatment have limited efficacy. 1,8-Dioxooctahydroxanthenes and tetraketones are compounds with important biological applications. The aim of this study was to assess the trypanocidal and inflammatory activities of nine 1,8-dioxooctahydroxanthenes (1–9) and three tetraketones (10–12). Methods and results: By in vitro killing assay, three compounds were able to eliminate CL TdTomato expressing strain of T. cruzi, 9 (IC50 = 30.65 μM), 10 (IC50 = 14.11 μM), and 11 (IC50 = 26.43 μM). However, only 9 was not toxic to Vero cells. Next, to evaluate the in vivo antitrypanosomal and immunological efficacy of 9, Swiss mice were infected with the Y and CL strains of T. cruzi and treated for 10 days with 50 mg/kg of 9. This compound reduced the cardiac inflammatory infiltration in animals infected with both strains. Rank's ligand (RankL), CCL2, and interferon (IFN)-γ were measured in the cardiac tissue homogenate of the Y-strain-infected animals, and no interference of 9 was observed. However, compound 9 increased the RankL and interleukin (IL)-10 levels in CL-infected mice. No hepatic and renal toxicity was observed. Conclusion: Our findings showed that 1,8-dioxooctahydroxanthene has antiparasitic effect and ameliorates the cardiac inflammatory parameters related to T. cruzi infection. |
topic |
Trypanosoma cruzi Xanthenodiones Tetraketones Inflammation Heart damage Benznidazole |
url |
http://www.sciencedirect.com/science/article/pii/S2213716520300977 |
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