EPA/DHA Concentrate by Urea Complexation Decreases Hyperinsulinemia and Increases Plin5 in the Liver of Mice Fed a High-Fat Diet
<b>: </b>Dietary intake of eicosapentaenoic/docosahexaenoic acid (EPA/DHA) reduces insulin resistance and hepatic manifestations through the regulation of metabolism in the liver. Obese mice present insulin resistance and lipid accumulation in intracellular lipid droplets (LDs). LD-assoc...
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doaj-b1e2fb38b862448bb1cfcc039c08f7c52020-11-25T03:44:44ZengMDPI AGMolecules1420-30492020-07-01253289328910.3390/molecules25143289EPA/DHA Concentrate by Urea Complexation Decreases Hyperinsulinemia and Increases Plin5 in the Liver of Mice Fed a High-Fat DietAlejandra Espinosa0Andrés Ross1Gretel Dovale-Rosabal2Francisco Pino-de la Fuente3Ernesto Uribe-Oporto4Camila Sacristán5Paulina Ruiz6Rodrigo Valenzuela7Nalda Romero8Santiago P. Aubourg9Alicia Rodríguez10Department of Medical Technology, Faculty of Medicine, University of Chile, 8380000 Santiago, ChileDepartment of Food Science and Chemical Technology, Faculty of Chemical and Pharmaceutical Sciences, University of Chile, Santos Dumont 964, 8380494 Santiago, ChileDepartment of Food Science and Chemical Technology, Faculty of Chemical and Pharmaceutical Sciences, University of Chile, Santos Dumont 964, 8380494 Santiago, ChileDepartment of Medical Technology, Faculty of Medicine, University of Chile, 8380000 Santiago, ChileDepartment of Medical Technology, Faculty of Medicine, University of Chile, 8380000 Santiago, ChileDepartment of Medical Technology, Faculty of Medicine, University of Chile, 8380000 Santiago, ChileDepartment of Medical Technology, Faculty of Medicine, University of Chile, 8380000 Santiago, ChileNutrition Department, Faculty of Medicine, University of Chile, 8380000 Santiago, ChileDepartment of Food Science and Chemical Technology, Faculty of Chemical and Pharmaceutical Sciences, University of Chile, Santos Dumont 964, 8380494 Santiago, ChileDepartment of Food Technology, Marine Research Institute (CSIC), Eduardo Cabello, 6, 36208 Vigo, SpainDepartment of Food Science and Chemical Technology, Faculty of Chemical and Pharmaceutical Sciences, University of Chile, Santos Dumont 964, 8380494 Santiago, Chile<b>: </b>Dietary intake of eicosapentaenoic/docosahexaenoic acid (EPA/DHA) reduces insulin resistance and hepatic manifestations through the regulation of metabolism in the liver. Obese mice present insulin resistance and lipid accumulation in intracellular lipid droplets (LDs). LD-associated proteins perilipin (Plin) have an essential role in both adipogenesis and lipolysis; Plin5 regulates lipolysis and thus contributes to fat oxidation. The purpose of this study was to compare the effects of deodorized refined salmon oil (DSO) and its polyunsaturated fatty acids concentrate (CPUFA) containing EPA and DHA, obtained by complexing with urea, on obesity-induced metabolic alteration. CPUFA maximum content was determined using the Box–Behnken experimental design based on Surface Response Methodology. The optimized CPUFA was administered to high-fat diet (HFD)-fed mice (200 mg/kg/day of EPA + DHA) for 8 weeks. No significant differences (<i>p</i><i> </i>> 0.05) in cholesterol, glycemia, LDs or transaminase content were found. Fasting insulin and hepatic Plin5 protein level increased in the group supplemented with the EPA + DHA optimized product (38.35 g/100 g total fatty acids) compared to obese mice without fish oil supplementation. The results suggest that processing salmon oil by urea concentration can generate an EPA+DHA dose useful to prevent the increase of fasting insulin and the decrease of Plin5 in the liver of insulin-resistant mice.https://www.mdpi.com/1420-3049/25/14/3289deodorized refined salmon oilurea complexationEPA + DHA concentrationmetabolic alterationsinsulinobesity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alejandra Espinosa Andrés Ross Gretel Dovale-Rosabal Francisco Pino-de la Fuente Ernesto Uribe-Oporto Camila Sacristán Paulina Ruiz Rodrigo Valenzuela Nalda Romero Santiago P. Aubourg Alicia Rodríguez |
spellingShingle |
Alejandra Espinosa Andrés Ross Gretel Dovale-Rosabal Francisco Pino-de la Fuente Ernesto Uribe-Oporto Camila Sacristán Paulina Ruiz Rodrigo Valenzuela Nalda Romero Santiago P. Aubourg Alicia Rodríguez EPA/DHA Concentrate by Urea Complexation Decreases Hyperinsulinemia and Increases Plin5 in the Liver of Mice Fed a High-Fat Diet Molecules deodorized refined salmon oil urea complexation EPA + DHA concentration metabolic alterations insulin obesity |
author_facet |
Alejandra Espinosa Andrés Ross Gretel Dovale-Rosabal Francisco Pino-de la Fuente Ernesto Uribe-Oporto Camila Sacristán Paulina Ruiz Rodrigo Valenzuela Nalda Romero Santiago P. Aubourg Alicia Rodríguez |
author_sort |
Alejandra Espinosa |
title |
EPA/DHA Concentrate by Urea Complexation Decreases Hyperinsulinemia and Increases Plin5 in the Liver of Mice Fed a High-Fat Diet |
title_short |
EPA/DHA Concentrate by Urea Complexation Decreases Hyperinsulinemia and Increases Plin5 in the Liver of Mice Fed a High-Fat Diet |
title_full |
EPA/DHA Concentrate by Urea Complexation Decreases Hyperinsulinemia and Increases Plin5 in the Liver of Mice Fed a High-Fat Diet |
title_fullStr |
EPA/DHA Concentrate by Urea Complexation Decreases Hyperinsulinemia and Increases Plin5 in the Liver of Mice Fed a High-Fat Diet |
title_full_unstemmed |
EPA/DHA Concentrate by Urea Complexation Decreases Hyperinsulinemia and Increases Plin5 in the Liver of Mice Fed a High-Fat Diet |
title_sort |
epa/dha concentrate by urea complexation decreases hyperinsulinemia and increases plin5 in the liver of mice fed a high-fat diet |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2020-07-01 |
description |
<b>: </b>Dietary intake of eicosapentaenoic/docosahexaenoic acid (EPA/DHA) reduces insulin resistance and hepatic manifestations through the regulation of metabolism in the liver. Obese mice present insulin resistance and lipid accumulation in intracellular lipid droplets (LDs). LD-associated proteins perilipin (Plin) have an essential role in both adipogenesis and lipolysis; Plin5 regulates lipolysis and thus contributes to fat oxidation. The purpose of this study was to compare the effects of deodorized refined salmon oil (DSO) and its polyunsaturated fatty acids concentrate (CPUFA) containing EPA and DHA, obtained by complexing with urea, on obesity-induced metabolic alteration. CPUFA maximum content was determined using the Box–Behnken experimental design based on Surface Response Methodology. The optimized CPUFA was administered to high-fat diet (HFD)-fed mice (200 mg/kg/day of EPA + DHA) for 8 weeks. No significant differences (<i>p</i><i> </i>> 0.05) in cholesterol, glycemia, LDs or transaminase content were found. Fasting insulin and hepatic Plin5 protein level increased in the group supplemented with the EPA + DHA optimized product (38.35 g/100 g total fatty acids) compared to obese mice without fish oil supplementation. The results suggest that processing salmon oil by urea concentration can generate an EPA+DHA dose useful to prevent the increase of fasting insulin and the decrease of Plin5 in the liver of insulin-resistant mice. |
topic |
deodorized refined salmon oil urea complexation EPA + DHA concentration metabolic alterations insulin obesity |
url |
https://www.mdpi.com/1420-3049/25/14/3289 |
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