Fluorescent protein tagging as a tool to define the subcellular distribution of proteins in plants

Fluorescent protein (FP) tagging approaches are widely used to determine the subcellular location of plant proteins. Here we give a brief overview of FP approaches, highlight potential technical problems, and discuss what to consider when designing FP/protein fusion constructs and performing transfo...

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Bibliographic Details
Main Authors: Sandra Karin Tanz, Ian eCastleden, Ian D. Small, A. Harvey eMillar
Format: Article
Language:English
Published: Frontiers Media S.A. 2013-06-01
Series:Frontiers in Plant Science
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00214/full