Dissection of goadsporin biosynthesis by in vitro reconstitution leading to designer analogues expressed in vivo

Goadsporin is an antibacterial peptide highly customized by multiple post-translational modifying enzymes. Here, the authors dissect its biosynthetic pathway byin vitro reconstitution and use their insights to produce goadsporin analogues in vitro and in vivo.

Bibliographic Details
Main Authors:	Taro Ozaki, Kona Yamashita, Yuki Goto, Morito Shimomura, Shohei Hayashi, Shumpei Asamizu, Yoshinori Sugai, Haruo Ikeda, Hiroaki Suga, Hiroyasu Onaka
Format:	Article
Language:	English
Published:	Nature Publishing Group 2017-02-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/ncomms14207

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spelling	doaj-b21b652ae3eb468b9440c69d702053172021-05-11T07:38:37ZengNature Publishing GroupNature Communications2041-17232017-02-018111310.1038/ncomms14207Dissection of goadsporin biosynthesis by in vitro reconstitution leading to designer analogues expressed in vivoTaro Ozaki0Kona Yamashita1Yuki Goto2Morito Shimomura3Shohei Hayashi4Shumpei Asamizu5Yoshinori Sugai6Haruo Ikeda7Hiroaki Suga8Hiroyasu Onaka9Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of TokyoDepartment of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of TokyoDepartment of Chemistry, Graduate School of Science, The University of TokyoDepartment of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of TokyoDepartment of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of TokyoDepartment of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of TokyoDepartment of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of TokyoKitasato Institute for Life Sciences, Kitasato UniversityDepartment of Chemistry, Graduate School of Science, The University of TokyoDepartment of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of TokyoGoadsporin is an antibacterial peptide highly customized by multiple post-translational modifying enzymes. Here, the authors dissect its biosynthetic pathway byin vitro reconstitution and use their insights to produce goadsporin analogues in vitro and in vivo.https://doi.org/10.1038/ncomms14207
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Taro Ozaki Kona Yamashita Yuki Goto Morito Shimomura Shohei Hayashi Shumpei Asamizu Yoshinori Sugai Haruo Ikeda Hiroaki Suga Hiroyasu Onaka
spellingShingle	Taro Ozaki Kona Yamashita Yuki Goto Morito Shimomura Shohei Hayashi Shumpei Asamizu Yoshinori Sugai Haruo Ikeda Hiroaki Suga Hiroyasu Onaka Dissection of goadsporin biosynthesis by in vitro reconstitution leading to designer analogues expressed in vivo Nature Communications
author_facet	Taro Ozaki Kona Yamashita Yuki Goto Morito Shimomura Shohei Hayashi Shumpei Asamizu Yoshinori Sugai Haruo Ikeda Hiroaki Suga Hiroyasu Onaka
author_sort	Taro Ozaki
title	Dissection of goadsporin biosynthesis by in vitro reconstitution leading to designer analogues expressed in vivo
title_short	Dissection of goadsporin biosynthesis by in vitro reconstitution leading to designer analogues expressed in vivo
title_full	Dissection of goadsporin biosynthesis by in vitro reconstitution leading to designer analogues expressed in vivo
title_fullStr	Dissection of goadsporin biosynthesis by in vitro reconstitution leading to designer analogues expressed in vivo
title_full_unstemmed	Dissection of goadsporin biosynthesis by in vitro reconstitution leading to designer analogues expressed in vivo
title_sort	dissection of goadsporin biosynthesis by in vitro reconstitution leading to designer analogues expressed in vivo
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2017-02-01
description	Goadsporin is an antibacterial peptide highly customized by multiple post-translational modifying enzymes. Here, the authors dissect its biosynthetic pathway byin vitro reconstitution and use their insights to produce goadsporin analogues in vitro and in vivo.
url	https://doi.org/10.1038/ncomms14207
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Dissection of goadsporin biosynthesis by in vitro reconstitution leading to designer analogues expressed in vivo

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