Rapid assembly of customized TALENs into multiple delivery systems.

Transcriptional activator-like effector nucleases (TALENs) have become a powerful tool for genome editing. Here we present an efficient TALEN assembly approach in which TALENs are assembled by direct Golden Gate ligation into Gateway(®) Entry vectors from a repeat variable di-residue (RVD) plasmid a...

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Bibliographic Details
Main Authors: Zhengxing Zhang, Siliang Zhang, Xin Huang, Kyle E Orwig, Yi Sheng
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3820630?pdf=render