A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a1, MSA-2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-γ producing cells
Abstract Background Babesia bovis is a tick-transmitted protozoan hemoparasite and the causative agent of bovine babesiosis, a potential risk to more than 500 million cattle worldwide. The vaccines currently available are based on attenuated parasites, which are difficult to produce, and are only re...
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doaj-b2452397f4b24829b548625b537b011d2020-11-24T22:17:01ZengBMCParasites & Vectors1756-33052016-11-019111310.1186/s13071-016-1862-1A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a1, MSA-2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-γ producing cellsAlba Marina Gimenez0Katia S. Françoso1Jonatan Ersching2Marcelo Y. Icimoto3Vitor Oliveira4Anabel E. Rodriguez5Leonhard Schnittger6Monica Florin-Christensen7Mauricio M. Rodrigues8Irene S. Soares9CTCMOL, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo-Escola Paulista de MedicinaDepartamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São PauloCTCMOL, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo-Escola Paulista de MedicinaDepartamento de Biofísica, Universidade Federal de São PauloDepartamento de Biofísica, Universidade Federal de São PauloInstituto de Patobiologia, CICVyA, INTA-CastelarInstituto de Patobiologia, CICVyA, INTA-CastelarInstituto de Patobiologia, CICVyA, INTA-CastelarCTCMOL, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo-Escola Paulista de MedicinaDepartamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São PauloAbstract Background Babesia bovis is a tick-transmitted protozoan hemoparasite and the causative agent of bovine babesiosis, a potential risk to more than 500 million cattle worldwide. The vaccines currently available are based on attenuated parasites, which are difficult to produce, and are only recommended for use in bovines under one year of age. When used in older animals, these vaccines may cause life-threatening clinical symptoms and eventually death. The development of a multi-subunit recombinant vaccine against B. bovis would be attractive from an economic standpoint and, most importantly, could be recommended for animals of any age. In the present study, recombinant ectodomains of MSA-2a1, MSA-2b and MSA-2c antigens were expressed in Pichia pastoris yeast as secreted soluble peptides. Results The antigens were purified to homogeneity, and biochemically and immunologically characterized. A vaccine formulation was obtained by emulsifying a mixture of the three peptides with the adjuvant Montanide ISA 720, which elicited high IgG antibody titers against each of the above antigens. IgG antibodies generated against each MSA-antigen recognized merozoites and significantly inhibited the invasion of bovine erythrocytes. Cellular immune responses were also detected, which were characterized by splenic and lymph node CD4+ T cells producing IFN-γ and TNF-α upon stimulation with the antigens MSA-2a1 or MSA-2c. Conclusions These data strongly suggest the high protective potential of the presented formulation, and we propose that it could be tested in vaccination trials of bovines challenged with B. bovis.http://link.springer.com/article/10.1186/s13071-016-1862-1Babesia bovisMerozoitesRecombinant vaccine |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alba Marina Gimenez Katia S. Françoso Jonatan Ersching Marcelo Y. Icimoto Vitor Oliveira Anabel E. Rodriguez Leonhard Schnittger Monica Florin-Christensen Mauricio M. Rodrigues Irene S. Soares |
spellingShingle |
Alba Marina Gimenez Katia S. Françoso Jonatan Ersching Marcelo Y. Icimoto Vitor Oliveira Anabel E. Rodriguez Leonhard Schnittger Monica Florin-Christensen Mauricio M. Rodrigues Irene S. Soares A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a1, MSA-2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-γ producing cells Parasites & Vectors Babesia bovis Merozoites Recombinant vaccine |
author_facet |
Alba Marina Gimenez Katia S. Françoso Jonatan Ersching Marcelo Y. Icimoto Vitor Oliveira Anabel E. Rodriguez Leonhard Schnittger Monica Florin-Christensen Mauricio M. Rodrigues Irene S. Soares |
author_sort |
Alba Marina Gimenez |
title |
A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a1, MSA-2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-γ producing cells |
title_short |
A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a1, MSA-2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-γ producing cells |
title_full |
A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a1, MSA-2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-γ producing cells |
title_fullStr |
A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a1, MSA-2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-γ producing cells |
title_full_unstemmed |
A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a1, MSA-2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-γ producing cells |
title_sort |
recombinant multi-antigen vaccine formulation containing babesia bovis merozoite surface antigens msa-2a1, msa-2b and msa-2c elicits invasion-inhibitory antibodies and ifn-γ producing cells |
publisher |
BMC |
series |
Parasites & Vectors |
issn |
1756-3305 |
publishDate |
2016-11-01 |
description |
Abstract Background Babesia bovis is a tick-transmitted protozoan hemoparasite and the causative agent of bovine babesiosis, a potential risk to more than 500 million cattle worldwide. The vaccines currently available are based on attenuated parasites, which are difficult to produce, and are only recommended for use in bovines under one year of age. When used in older animals, these vaccines may cause life-threatening clinical symptoms and eventually death. The development of a multi-subunit recombinant vaccine against B. bovis would be attractive from an economic standpoint and, most importantly, could be recommended for animals of any age. In the present study, recombinant ectodomains of MSA-2a1, MSA-2b and MSA-2c antigens were expressed in Pichia pastoris yeast as secreted soluble peptides. Results The antigens were purified to homogeneity, and biochemically and immunologically characterized. A vaccine formulation was obtained by emulsifying a mixture of the three peptides with the adjuvant Montanide ISA 720, which elicited high IgG antibody titers against each of the above antigens. IgG antibodies generated against each MSA-antigen recognized merozoites and significantly inhibited the invasion of bovine erythrocytes. Cellular immune responses were also detected, which were characterized by splenic and lymph node CD4+ T cells producing IFN-γ and TNF-α upon stimulation with the antigens MSA-2a1 or MSA-2c. Conclusions These data strongly suggest the high protective potential of the presented formulation, and we propose that it could be tested in vaccination trials of bovines challenged with B. bovis. |
topic |
Babesia bovis Merozoites Recombinant vaccine |
url |
http://link.springer.com/article/10.1186/s13071-016-1862-1 |
work_keys_str_mv |
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