Protocols for endothelial cell isolation from mouse tissues: small intestine, colon, heart, and liver

Protocols for endothelial cell isolation from mouse tissues: small intestine, colon, heart, and liver

Summary: Endothelial cells (ECs) from the small intestine, colon, liver, and heart have distinct phenotypes and functional adaptations that are dependent on their physiological environment. Gut ECs adapt to low oxygen, heart ECs to contractile forces, and liver ECs to low flow rates. Isolating high-...

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Bibliographic Details
Main Authors: Liliana Sokol, Vincent Geldhof, Melissa García-Caballero, Nadine V. Conchinha, Sébastien J. Dumas, Elda Meta, Laure-Anne Teuwen, Koen Veys, Rongyuan Chen, Lucas Treps, Mila Borri, Pauline de Zeeuw, Kim D. Falkenberg, Charlotte Dubois, Magdalena Parys, Laura P.M.H. de Rooij, Jermaine Goveia, Katerina Rohlenova, Luc Schoonjans, Mieke Dewerchin, Guy Eelen, Xuri Li, Joanna Kalucka, Peter Carmeliet
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Cell isolation
Single Cell
Flow Cytometry/Mass Cytometry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721001969

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http://www.sciencedirect.com/science/article/pii/S2666166721001969

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