A universal vector for high-efficiency multi-fragment recombineering of BACs and knock-in constructs.

A universal vector for high-efficiency multi-fragment recombineering of BACs and knock-in constructs.

There is an increasing need for more efficient generation of transgenic constructs. Here we present a universal multi-site Gateway vector for use in recombineering reactions. Using transgenic mouse models, we show its use for the generation of BAC transgenics and targeting vectors. The modular natur...

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Bibliographic Details
Main Authors: Karamjit Singh Dolt, Melanie L Lawrence, Eve Miller-Hodges, Joan Slight, Anna Thornburn, Paul S Devenney, Peter Hohenstein
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3639282?pdf=render

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http://europepmc.org/articles/PMC3639282?pdf=render

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