Detection and quantification of γ-H2AX using a dissociation enhanced lanthanide fluorescence immunoassay

Detection and quantification of γ-H2AX using a dissociation enhanced lanthanide fluorescence immunoassay

Abstract Phosphorylation of the histone protein H2AX to form γ-H2AX foci directly represents DNA double-strand break formation. Traditional γ-H2AX detection involves counting individual foci within individual nuclei. The novelty of this work is the application of a time-resolved fluorescence assay u...

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Bibliographic Details
Main Authors: Felicite K. Noubissi, Amber A. McBride, Hannah G. Leppert, Larry J. Millet, Xiaofei Wang, Sandra M. Davern
Format: Article
Language:English
Published: Nature Publishing Group 2021-04-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-88296-3

Internet

https://doi.org/10.1038/s41598-021-88296-3

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