Growth hormone upregulates the pro-tumorigenic galectin 1 in mouse liver
Transgenic mice overexpressing growth hormone (GH) spontaneously develop liver tumors, including hepatocellular carcinoma (HCC), within a year. The preneoplastic liver pathology in these mice recapitulates that observed in humans at high risk of developing hepatic cancer. Although increased expressi...
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Format: | Article |
Language: | English |
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Bioscientifica
2019-07-01
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Series: | Endocrine Connections |
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Online Access: | https://ec.bioscientifica.com/view/journals/ec/8/8/EC-19-0292.xml |
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doaj-b33d034c83684217b8fa29d8cb688f3c |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
María L Bacigalupo Verónica G Piazza Nadia S Cicconi Pablo Carabias Andrzej Bartke Yimin Fang Ana I Sotelo Gabriel A Rabinovich María F Troncoso Johanna G Miquet |
spellingShingle |
María L Bacigalupo Verónica G Piazza Nadia S Cicconi Pablo Carabias Andrzej Bartke Yimin Fang Ana I Sotelo Gabriel A Rabinovich María F Troncoso Johanna G Miquet Growth hormone upregulates the pro-tumorigenic galectin 1 in mouse liver Endocrine Connections growth hormone galectin 1 hepatocellular carcinoma liver cancer |
author_facet |
María L Bacigalupo Verónica G Piazza Nadia S Cicconi Pablo Carabias Andrzej Bartke Yimin Fang Ana I Sotelo Gabriel A Rabinovich María F Troncoso Johanna G Miquet |
author_sort |
María L Bacigalupo |
title |
Growth hormone upregulates the pro-tumorigenic galectin 1 in mouse liver |
title_short |
Growth hormone upregulates the pro-tumorigenic galectin 1 in mouse liver |
title_full |
Growth hormone upregulates the pro-tumorigenic galectin 1 in mouse liver |
title_fullStr |
Growth hormone upregulates the pro-tumorigenic galectin 1 in mouse liver |
title_full_unstemmed |
Growth hormone upregulates the pro-tumorigenic galectin 1 in mouse liver |
title_sort |
growth hormone upregulates the pro-tumorigenic galectin 1 in mouse liver |
publisher |
Bioscientifica |
series |
Endocrine Connections |
issn |
2049-3614 2049-3614 |
publishDate |
2019-07-01 |
description |
Transgenic mice overexpressing growth hormone (GH) spontaneously develop liver tumors, including hepatocellular carcinoma (HCC), within a year. The preneoplastic liver pathology in these mice recapitulates that observed in humans at high risk of developing hepatic cancer. Although increased expression of galectin 1 (GAL1) in liver tissue is associated with HCC aggressiveness, a link between this glycan-binding protein and hormone-related tumor development has not yet been explored. In this study, we investigated GAL1 expression during liver tumor progression in mice continuously exposed to high levels of GH. GAL1 expression was determined by Western blotting, RT-qPCR and immunohistochemistry in the liver of transgenic mice overexpressing GH. Animals of representative ages at different stages of liver pathology were studied. GAL1 expression was upregulated in the liver of GH-transgenic mice. This effect was observed at early ages, when animals displayed no signs of liver disease or minimal histopathological alterations and was also detected in young adults with preneoplastic liver pathology. Remarkably, GAL1 upregulation was sustained during aging and its expression was particularly enhanced in liver tumors. GH also induced hepatic GAL1 expression in mice that were treated with this hormone for a short period. Moreover, GH triggered a rapid increment in GAL1 protein expression in human HCC cells, denoting a direct effect of the hormone on hepatocytes. Therefore, our results indicate that GH upregulates GAL1 expression in mouse liver, which may have critical implications in tumorigenesis. These findings suggest that this lectin could be implicated in hormone-driven liver carcinogenesis. |
topic |
growth hormone galectin 1 hepatocellular carcinoma liver cancer |
url |
https://ec.bioscientifica.com/view/journals/ec/8/8/EC-19-0292.xml |
work_keys_str_mv |
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spelling |
doaj-b33d034c83684217b8fa29d8cb688f3c2020-11-25T01:52:00ZengBioscientificaEndocrine Connections2049-36142049-36142019-07-018811081117https://doi.org/10.1530/EC-19-0292Growth hormone upregulates the pro-tumorigenic galectin 1 in mouse liverMaría L Bacigalupo0Verónica G Piazza1Nadia S Cicconi2Pablo Carabias3Andrzej Bartke4Yimin Fang5Ana I Sotelo6Gabriel A Rabinovich7María F Troncoso8Johanna G Miquet9Departamento de Química Biológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Química y Fisicoquímica Biológicas, Buenos Aires, ArgentinaDepartamento de Química Biológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Química y Fisicoquímica Biológicas, Buenos Aires, ArgentinaDepartamento de Química Biológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Química y Fisicoquímica Biológicas, Buenos Aires, ArgentinaDepartamento de Química Biológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Química y Fisicoquímica Biológicas, Buenos Aires, ArgentinaDepartment of Internal Medicine, Geriatrics Research, Southern Illinois University School of Medicine, Springfield, Illinois, USADepartment of Internal Medicine, Geriatrics Research, Southern Illinois University School of Medicine, Springfield, Illinois, USADepartamento de Química Biológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Química y Fisicoquímica Biológicas, Buenos Aires, ArgentinaLaboratorio de Inmunopatología, Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, and Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, ArgentinaDepartamento de Química Biológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Química y Fisicoquímica Biológicas, Buenos Aires, ArgentinaDepartamento de Química Biológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Química y Fisicoquímica Biológicas, Buenos Aires, ArgentinaTransgenic mice overexpressing growth hormone (GH) spontaneously develop liver tumors, including hepatocellular carcinoma (HCC), within a year. The preneoplastic liver pathology in these mice recapitulates that observed in humans at high risk of developing hepatic cancer. Although increased expression of galectin 1 (GAL1) in liver tissue is associated with HCC aggressiveness, a link between this glycan-binding protein and hormone-related tumor development has not yet been explored. In this study, we investigated GAL1 expression during liver tumor progression in mice continuously exposed to high levels of GH. GAL1 expression was determined by Western blotting, RT-qPCR and immunohistochemistry in the liver of transgenic mice overexpressing GH. Animals of representative ages at different stages of liver pathology were studied. GAL1 expression was upregulated in the liver of GH-transgenic mice. This effect was observed at early ages, when animals displayed no signs of liver disease or minimal histopathological alterations and was also detected in young adults with preneoplastic liver pathology. Remarkably, GAL1 upregulation was sustained during aging and its expression was particularly enhanced in liver tumors. GH also induced hepatic GAL1 expression in mice that were treated with this hormone for a short period. Moreover, GH triggered a rapid increment in GAL1 protein expression in human HCC cells, denoting a direct effect of the hormone on hepatocytes. Therefore, our results indicate that GH upregulates GAL1 expression in mouse liver, which may have critical implications in tumorigenesis. These findings suggest that this lectin could be implicated in hormone-driven liver carcinogenesis.https://ec.bioscientifica.com/view/journals/ec/8/8/EC-19-0292.xmlgrowth hormonegalectin 1hepatocellular carcinomaliver cancer |