Digital droplet PCR accurately quantifies SARS-CoV-2 viral load from crude lysate without nucleic acid purification

Digital droplet PCR accurately quantifies SARS-CoV-2 viral load from crude lysate without nucleic acid purification

Abstract The COVID-19 pandemic caused by the SARS-CoV-2 virus motivates diverse diagnostic approaches due to the novel causative pathogen, incompletely understood clinical sequelae, and limited availability of testing resources. Given the variability in viral load across and within patients, absolut...

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Bibliographic Details
Main Authors: Harish N. Vasudevan, Peng Xu, Venice Servellita, Steve Miller, Leqian Liu, Allan Gopez, Charles Y. Chiu, Adam R. Abate
Format: Article
Language:English
Published: Nature Publishing Group 2021-01-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-020-80715-1

Internet

https://doi.org/10.1038/s41598-020-80715-1

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