Isolation and characterization of a serine proteinase with thrombin-like activity from the venom of the snake Bothrops asper

A serine proteinase with thrombin-like activity was isolated from the venom of the Central American pit viper Bothrops asper. Isolation was performed by a combination of affinity chromatography on aminobenzamidine-Sepharose and ion-exchange chromatography on DEAE-Sepharose. The enzyme accounts for a...

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Bibliographic Details
Main Authors: A.V Pérez, A Rucavado, L Sanz, J.J Calvete, J.M Gutiérrez
Format: Article
Language:English
Published: Associação Brasileira de Divulgação Científica 2008-01-01
Series:Brazilian Journal of Medical and Biological Research
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000100003