Live-cell imaging of circadian clock protein dynamics in CRISPR-generated knock-in cells
Live-cell recordings have been an important tool for studying circadian rhythms. Here the authors use CRISPR gene editing mediated knock-in to fluorescently tag Per2 and Cry1, and study cellular circadian dynamics of these two clock proteins.
Main Authors: | , , , , , , , , , , , , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2021-06-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-021-24086-9 |