Systematic evaluation of CRISPR-Cas systems reveals design principles for genome editing in human cells

Systematic evaluation of CRISPR-Cas systems reveals design principles for genome editing in human cells

Abstract Background While CRISPR-Cas systems hold tremendous potential for engineering the human genome, it is unclear how well each system performs against one another in both non-homologous end joining (NHEJ)-mediated and homology-directed repair (HDR)-mediated genome editing. Results We systemati...

Full description

Bibliographic Details
Main Authors: Yuanming Wang, Kaiwen Ivy Liu, Norfala-Aliah Binte Sutrisnoh, Harini Srinivasan, Junyi Zhang, Jia Li, Fan Zhang, Charles Richard John Lalith, Heyun Xing, Raghuvaran Shanmugam, Jia Nee Foo, Hwee Ting Yeo, Kean Hean Ooi, Tore Bleckwehl, Yi Yun Rachel Par, Shi Mun Lee, Nur Nadiah Binte Ismail, Nur Aidah Binti Sanwari, Si Ting Vanessa Lee, Jan Lew, Meng How Tan
Format: Article
Language:English
Published: BMC 2018-05-01
Series:Genome Biology
Subjects:
CRISPR
Genome editing
Non-homologous end joining (NHEJ)
Homology-directed repair (HDR)
Cas9 nucleases
Cpf1 nucleases
Online Access:http://link.springer.com/article/10.1186/s13059-018-1445-x

Internet

http://link.springer.com/article/10.1186/s13059-018-1445-x

Similar Items