Generation of an INSULIN-H2B-Cherry reporter human iPSC line

Differentiating human induced pluripotent stem cells (hiPSCs) into insulin (INS)-producing β-like cells has potential for diabetes research and therapy. Here, we generated a heterozygous fluorescent hiPSC reporter, labeling INS-producing β-like cells. We used CRISPR/Cas9 technology to knock-in a T2A...

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Bibliographic Details
Main Authors: Anna Karolina Blöchinger, Johanna Siehler, Katharina Wißmiller, Alireza Shahryari, Ingo Burtscher, Heiko Lickert
Format: Article
Language:English
Published: Elsevier 2020-05-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506120301008