Isolation of nuclei from mouse white adipose tissues for single-nucleus genomics

Isolation of nuclei from mouse white adipose tissues for single-nucleus genomics

Summary: Lipid-filled adipocytes are incompatible with droplet-based single-cell methods, such as 10x Genomics-based technology, thus restricting droplet-based single-cell analyses of adipose tissues to the stromal vascular fraction. To overcome this limitation and obtain cellular and molecular insi...

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Bibliographic Details
Main Authors: Elvira Laila Van Hauwaert, Ellen Gammelmark, Anitta Kinga Sárvári, Lena Larsen, Ronni Nielsen, Jesper Grud Skat Madsen, Susanne Mandrup
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Cell isolation
Single Cell
Genomics
RNA-seq
Metabolism
Gene Expression
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721003191

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http://www.sciencedirect.com/science/article/pii/S2666166721003191

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