A Novel Method to Titrate Herpes Simplex Virus-1 (HSV-1) Using Laser-Based Scanning of Near-Infrared Fluorophores Conjugated Antibodies

A Novel Method to Titrate Herpes Simplex Virus-1 (HSV-1) Using Laser-Based Scanning of Near-Infrared Fluorophores Conjugated Antibodies

Among several strategies used for Herpes simplex virus (HSV) detection in biological specimens, standard plaque assay (SPA) remains the most reliable method to evaluate virus infectivity and quantify viral replication. However, it is a manual procedure, thereby affected by operator subjectivity, and...

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Bibliographic Details
Main Authors: Marco Fabiani, Dolores Limongi, Anna Teresa Palamara, Giovanna De Chiara, Maria Elena Marcocci
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-06-01
Series:Frontiers in Microbiology
Subjects:
HSV-1
herpes simplex virus
plaque assay
virus titration
near-infrared fluorescence
immunostaining
Online Access:http://journal.frontiersin.org/article/10.3389/fmicb.2017.01085/full

Internet

http://journal.frontiersin.org/article/10.3389/fmicb.2017.01085/full

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