Programmed genome editing of the omega-1 ribonuclease of the blood fluke, Schistosoma mansoni

Programmed genome editing of the omega-1 ribonuclease of the blood fluke, Schistosoma mansoni

CRISPR/Cas9-based genome editing has yet to be reported in species of the Platyhelminthes. We tested this approach by targeting omega-1 (ω1) of Schistosoma mansoni as proof of principle. This secreted ribonuclease is crucial for Th2 polarization and granuloma formation. Schistosome eggs were exposed...

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Bibliographic Details
Main Authors: Wannaporn Ittiprasert, Victoria H Mann, Shannon E Karinshak, Avril Coghlan, Gabriel Rinaldi, Geetha Sankaranarayanan, Apisit Chaidee, Toshihiko Tanno, Chutima Kumkhaek, Pannathee Prangtaworn, Margaret M Mentink-Kane, Christina J Cochran, Patrick Driguez, Nancy Holroyd, Alan Tracey, Rutchanee Rodpai, Bart Everts, Cornelis H Hokke, Karl F Hoffmann, Matthew Berriman, Paul J Brindley
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2019-01-01
Series:eLife
Subjects:
Schistosoma mansoni
CRISPR/Cas9
omega-1
ribonuclease
granuloma
Th2-polarization
Online Access:https://elifesciences.org/articles/41337

Internet

https://elifesciences.org/articles/41337

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