AbSeq Protocol Using the Nano-Well Cartridge-Based Rhapsody Platform to Generate Protein and Transcript Expression Data on the Single-Cell Level

AbSeq Protocol Using the Nano-Well Cartridge-Based Rhapsody Platform to Generate Protein and Transcript Expression Data on the Single-Cell Level

Summary: By including oligonucleotide-labeled antibodies into high-throughput single-cell RNA-sequencing protocols, combined transcript and protein expression data can be acquired on the single-cell level. Here, we describe a protocol for the combined analysis of over 40 proteins and 400 genes on ov...

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Bibliographic Details
Main Authors: Jami R. Erickson, Florian Mair, Grace Bugos, Jody Martin, Aaron J. Tyznik, Margaret Nakamoto, Stefanie Mortimer, Martin Prlic
Format: Article
Language:English
Published: Elsevier 2020-09-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720300794

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http://www.sciencedirect.com/science/article/pii/S2666166720300794

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