A Linkage-specific Sialic Acid Labeling Strategy Reveals Different Site-specific Glycosylation Patterns in SARS-CoV-2 Spike Protein Produced in CHO and HEK Cell Substrates

A Linkage-specific Sialic Acid Labeling Strategy Reveals Different Site-specific Glycosylation Patterns in SARS-CoV-2 Spike Protein Produced in CHO and HEK Cell Substrates

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus utilizes the extensively glycosylated spike (S) protein protruding from the viral envelope to bind to angiotensin-converting enzyme-related carboxypeptidase (ACE2) as its primary receptor to mediate host-cell entry. Currently, th...

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Bibliographic Details
Main Authors: Qiong Wang, Yan Wang, Shuang Yang, Changyi Lin, Lateef Aliyu, Yiqun Chen, Lisa Parsons, Yuan Tian, Hongpeng Jia, Andrew Pekosz, Michael J. Betenbaugh, John F. Cipollo
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-09-01
Series:Frontiers in Chemistry
Subjects:
sialic acid
amidation
SARS-CoV-2
glycoproteomics
site occupancy
N-glycosylation
Online Access:https://www.frontiersin.org/articles/10.3389/fchem.2021.735558/full

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https://www.frontiersin.org/articles/10.3389/fchem.2021.735558/full

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