Development and Multiplexed Amplification of SSR Markers for <i>Thuja occidentalis</i> (Cupressaceae) Using Shotgun Pyrosequencing

Development and Multiplexed Amplification of SSR Markers for <i>Thuja occidentalis</i> (Cupressaceae) Using Shotgun Pyrosequencing

Premise of the study: Sixteen novel, polymorphic, multiplexed microsatellite loci were developed for eastern white cedar (<i>Thuja occidentalis</i>) using simple sequence repeat (SSR)–enriched shotgun pyrosequencing. Methods and Results: Sixteen loci were tested on a panel of 24 indiv...

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Bibliographic Details
Main Authors: Huaitong Xu, Francine Tremblay, Yves Bergeron
Format: Article
Language:English
Published: Wiley 2013-05-01
Series:Applications in Plant Sciences
Subjects:
454 GS-FLX Titanium
microsatellite marker
next-generation sequencing
population genetics
shotgun pyrosequencing
Thuja occidentalis
Online Access:http://www.bioone.org/doi/full/10.3732/apps.1200427

Internet

http://www.bioone.org/doi/full/10.3732/apps.1200427

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