A Versatile Strategy to Reduce UGA-Selenocysteine Recoding Efficiency of the Ribosome Using CRISPR-Cas9-Viral-Like-Particles Targeting Selenocysteine-tRNA<sup>[Ser]Sec</sup> Gene

A Versatile Strategy to Reduce UGA-Selenocysteine Recoding Efficiency of the Ribosome Using CRISPR-Cas9-Viral-Like-Particles Targeting Selenocysteine-tRNA<sup>[Ser]Sec</sup> Gene

The translation of selenoprotein mRNAs involves a non-canonical ribosomal event in which an in-frame UGA is recoded as a selenocysteine (Sec) codon instead of being read as a stop codon. The recoding machinery is centered around two dedicated RNA components: The selenocysteine insertion sequence (SE...

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Bibliographic Details
Main Authors: Caroline Vindry, Olivia Guillin, Philippe E. Mangeot, Théophile Ohlmann, Laurent Chavatte
Format: Article
Language:English
Published: MDPI AG 2019-06-01
Series:Cells
Subjects:
selenium
selenocysteine
selenoprotein
SECIS
Sec-tRNA<sup>[Ser]Sec</sup>
UGA-recoding
CRISPR-Cas9
viral-like particles
nanoblades
Online Access:https://www.mdpi.com/2073-4409/8/6/574

Internet

https://www.mdpi.com/2073-4409/8/6/574

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