SIFAT ANTAGONISTIK Lactobacillus sp B441 DAN II442 ASAL TEMPOYAK TERHADAP Staphylococcus aureus (Antagonism of Lactobacillus sp B441 and II442 from Tempoyak against Staphylococcus aureus)
Lactobacillus sp B441 and II442 were species of lactic acid bacteria isolated from tempoyak, a kind of food fermented made from durian flesh. Staphylococcus aureus is a pathogenic bacteria that has natural habitat on the surface of human body such as on the skin and in mucous membranes like the nose...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Universitas Gadjah Mada
2015-02-01
|
Series: | Agritech |
Online Access: | https://jurnal.ugm.ac.id/agritech/article/view/9438 |
id |
doaj-b52fcdf6d198420c94ca4e534999d2e5 |
---|---|
record_format |
Article |
spelling |
doaj-b52fcdf6d198420c94ca4e534999d2e52020-11-25T00:13:51ZengUniversitas Gadjah MadaAgritech0216-04552527-38252015-02-01340443043810.22146/agritech.94387608SIFAT ANTAGONISTIK Lactobacillus sp B441 DAN II442 ASAL TEMPOYAK TERHADAP Staphylococcus aureus (Antagonism of Lactobacillus sp B441 and II442 from Tempoyak against Staphylococcus aureus)Tri Wardani WidowatiBasuni HamzahAgus WijayaRindit PambayunLactobacillus sp B441 and II442 were species of lactic acid bacteria isolated from tempoyak, a kind of food fermented made from durian flesh. Staphylococcus aureus is a pathogenic bacteria that has natural habitat on the surface of human body such as on the skin and in mucous membranes like the nose. Contamination of Staphylococcus aureus was a risk factor in tempoyak fermentation, especially, during taking the durian flesh from seeds. The aim of this research was to observe antagonistic property of Lactobacillus sp B441 and II442 isolated from tempoyak during low temperature fermentation against Staphylococcus aureus. Phenotypic identification using API 50 CHL test kit indicated that isolates were Lactobacullus plantarum B441 and Lactobacillus plantarum II442. Based on co-culture analysis show that isolates of Lactobacillus plantarum B441 and II442 have bactericidal effect against Staphylococcus aureus ATCC 25923 as a indicator of pathogenic bacteria. At 25 th hours, population of Staphylococcus aureus ATCC 25923 in mix-culture with Lactobacillus plantarum B441 and Lactobacillus plantarum II442 decreased 3 – 4 log cycle from initial population. However, population of Staphylococcus aureus ATCC 25923 in mono-culture increased 2 log cycles at 25 th hours. Keywords: Lactobacillus sp, tempoyak, antagonism, Staphylococcus aureus ABSTRAK Lactobacillus sp B441 dan II442 merupakan spesies bakteri asam laktat yang diisolasi dari tempoyak yaitu makanan fermentasi dari daging buah durian. Staphylococcus aureus adalah bakteri patogenik yang habitat alaminya pada permukaan kulit manusia dan membran mukosa seperti hidung. Proses fermentasi tempoyak khususnya saat pemisahan daging buah durian dari bijinya beresiko terkontaminasi oleh Staphylococcus aureus. Tujuan dari penelitian ini adalah mengkaji sifat antagonistik dari Lactobacillus sp B441 and II442 yang diisolasi dari tempoyak terhadap Staphylococcus aureus. Identifikasi secara fenotif menggunakan API 50 CHL test kit menunjukkan bahwa isolat bakteri asam laktat tersebut adalah Lactobacullus plantarum B441 dan Lactobacillus plantarum II442. Berdasar uji co-culture diketahui bahwa kedua spesies tersebut mempunyai efek bakterisidal terhadap bakteri Staphylococcus aureus ATCC 25923. Pada jam ke 25 Staphylococcus aureus yang ditumbuhkan secara mix-culture dengan Lactobacillus plantarum B441 dan Lactobacillus plantarum II442 asal tempoyak turun 3 – 4 log cycle dari populasi awal. Sedangkan populasi Staphylococcus aureus ATCC 25923 secara mono-culture meningkat sekitar 2 log cycle pada jam ke 25. Kata kunci: Lactobacillus sp, tempoyak, antaginistik, Staphylococcus aureushttps://jurnal.ugm.ac.id/agritech/article/view/9438 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tri Wardani Widowati Basuni Hamzah Agus Wijaya Rindit Pambayun |
spellingShingle |
Tri Wardani Widowati Basuni Hamzah Agus Wijaya Rindit Pambayun SIFAT ANTAGONISTIK Lactobacillus sp B441 DAN II442 ASAL TEMPOYAK TERHADAP Staphylococcus aureus (Antagonism of Lactobacillus sp B441 and II442 from Tempoyak against Staphylococcus aureus) Agritech |
author_facet |
Tri Wardani Widowati Basuni Hamzah Agus Wijaya Rindit Pambayun |
author_sort |
Tri Wardani Widowati |
title |
SIFAT ANTAGONISTIK Lactobacillus sp B441 DAN II442 ASAL TEMPOYAK TERHADAP Staphylococcus aureus (Antagonism of Lactobacillus sp B441 and II442 from Tempoyak against Staphylococcus aureus) |
title_short |
SIFAT ANTAGONISTIK Lactobacillus sp B441 DAN II442 ASAL TEMPOYAK TERHADAP Staphylococcus aureus (Antagonism of Lactobacillus sp B441 and II442 from Tempoyak against Staphylococcus aureus) |
title_full |
SIFAT ANTAGONISTIK Lactobacillus sp B441 DAN II442 ASAL TEMPOYAK TERHADAP Staphylococcus aureus (Antagonism of Lactobacillus sp B441 and II442 from Tempoyak against Staphylococcus aureus) |
title_fullStr |
SIFAT ANTAGONISTIK Lactobacillus sp B441 DAN II442 ASAL TEMPOYAK TERHADAP Staphylococcus aureus (Antagonism of Lactobacillus sp B441 and II442 from Tempoyak against Staphylococcus aureus) |
title_full_unstemmed |
SIFAT ANTAGONISTIK Lactobacillus sp B441 DAN II442 ASAL TEMPOYAK TERHADAP Staphylococcus aureus (Antagonism of Lactobacillus sp B441 and II442 from Tempoyak against Staphylococcus aureus) |
title_sort |
sifat antagonistik lactobacillus sp b441 dan ii442 asal tempoyak terhadap staphylococcus aureus (antagonism of lactobacillus sp b441 and ii442 from tempoyak against staphylococcus aureus) |
publisher |
Universitas Gadjah Mada |
series |
Agritech |
issn |
0216-0455 2527-3825 |
publishDate |
2015-02-01 |
description |
Lactobacillus sp B441 and II442 were species of lactic acid bacteria isolated from tempoyak, a kind of food fermented made from durian flesh. Staphylococcus aureus is a pathogenic bacteria that has natural habitat on the surface of human body such as on the skin and in mucous membranes like the nose. Contamination of Staphylococcus aureus
was a risk factor in tempoyak fermentation, especially, during taking the durian flesh from seeds. The aim of this research was to observe antagonistic property of Lactobacillus sp B441 and II442 isolated from tempoyak during low temperature fermentation against Staphylococcus aureus. Phenotypic identification using API 50 CHL test kit indicated
that isolates were Lactobacullus plantarum B441 and Lactobacillus plantarum II442. Based on co-culture analysis show that isolates of Lactobacillus plantarum B441 and II442 have bactericidal effect against Staphylococcus aureus ATCC 25923 as a indicator of pathogenic bacteria. At 25 th hours, population of Staphylococcus aureus ATCC 25923 in mix-culture with Lactobacillus plantarum B441 and Lactobacillus plantarum II442 decreased 3 – 4 log cycle from initial population. However, population of Staphylococcus aureus ATCC 25923 in mono-culture increased 2 log cycles at 25 th hours.
Keywords: Lactobacillus sp, tempoyak, antagonism, Staphylococcus aureus
ABSTRAK
Lactobacillus sp B441 dan II442 merupakan spesies bakteri asam laktat yang diisolasi dari tempoyak yaitu makanan fermentasi dari daging buah durian. Staphylococcus aureus adalah bakteri patogenik yang habitat alaminya pada permukaan kulit manusia dan membran mukosa seperti hidung. Proses fermentasi tempoyak khususnya saat pemisahan
daging buah durian dari bijinya beresiko terkontaminasi oleh Staphylococcus aureus. Tujuan dari penelitian ini adalah mengkaji sifat antagonistik dari Lactobacillus sp B441 and II442 yang diisolasi dari tempoyak terhadap Staphylococcus aureus. Identifikasi secara fenotif menggunakan API 50 CHL test kit menunjukkan bahwa isolat bakteri asam laktat tersebut adalah Lactobacullus plantarum B441 dan Lactobacillus plantarum II442. Berdasar uji co-culture diketahui
bahwa kedua spesies tersebut mempunyai efek bakterisidal terhadap bakteri Staphylococcus aureus ATCC 25923. Pada jam ke 25 Staphylococcus aureus yang ditumbuhkan secara mix-culture dengan Lactobacillus plantarum B441 dan Lactobacillus plantarum II442 asal tempoyak turun 3 – 4 log cycle dari populasi awal. Sedangkan populasi
Staphylococcus aureus ATCC 25923 secara mono-culture meningkat sekitar 2 log cycle pada jam ke 25.
Kata kunci: Lactobacillus sp, tempoyak, antaginistik, Staphylococcus aureus |
url |
https://jurnal.ugm.ac.id/agritech/article/view/9438 |
work_keys_str_mv |
AT triwardaniwidowati sifatantagonistiklactobacillusspb441danii442asaltempoyakterhadapstaphylococcusaureusantagonismoflactobacillusspb441andii442fromtempoyakagainststaphylococcusaureus AT basunihamzah sifatantagonistiklactobacillusspb441danii442asaltempoyakterhadapstaphylococcusaureusantagonismoflactobacillusspb441andii442fromtempoyakagainststaphylococcusaureus AT aguswijaya sifatantagonistiklactobacillusspb441danii442asaltempoyakterhadapstaphylococcusaureusantagonismoflactobacillusspb441andii442fromtempoyakagainststaphylococcusaureus AT rinditpambayun sifatantagonistiklactobacillusspb441danii442asaltempoyakterhadapstaphylococcusaureusantagonismoflactobacillusspb441andii442fromtempoyakagainststaphylococcusaureus |
_version_ |
1725392875460493312 |