Highly sensitive quantification of key regulatory oxysterols in biological samples by LC-ESI-MS/MSs⃞

Highly sensitive quantification of key regulatory oxysterols in biological samples by LC-ESI-MS/MSs⃞

We describe a highly sensitive and specific method for the quantification of key regulatory oxysterols in biological samples. This method is based upon a stable isotope dilution technique by liquid chromatography-tandem mass spectrometry (LC-MS/MS). After alkaline hydrolysis of human serum (5 μl) or...

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Bibliographic Details
Main Authors: Akira Honda, Kouwa Yamashita, Takashi Hara, Tadashi Ikegami, Teruo Miyazaki, Mutsumi Shirai, Guorong Xu, Mitsuteru Numazawa, Yasushi Matsuzaki
Format: Article
Language:English
Published: Elsevier 2009-02-01
Series:Journal of Lipid Research
Subjects:
liquid chromatography-tandem mass spectrometry
electrospray ionization
24S,25-epoxycholesterol
4β-hydroxycholesterol
7α-hydroxycholesterol
22R-hydroxycholesterol
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520414592

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http://www.sciencedirect.com/science/article/pii/S0022227520414592

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