Identification of major factors influencing ELISpot-based monitoring of cellular responses to antigens from Mycobacterium tuberculosis.

A number of different interferon-gamma ELISpot protocols are in use in laboratories studying antigen-specific immune responses. It is therefore unclear how results from different assays compare, and what factors most significantly influence assay outcome. One such difference is that some laboratorie...

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Bibliographic Details
Main Authors: Steven G Smith, Simone A Joosten, Virginie Verscheure, Ansar A Pathan, Helen McShane, Tom H M Ottenhoff, Hazel M Dockrell, Françoise Mascart
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2009-11-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2776358?pdf=render