Identification of major factors influencing ELISpot-based monitoring of cellular responses to antigens from Mycobacterium tuberculosis.
A number of different interferon-gamma ELISpot protocols are in use in laboratories studying antigen-specific immune responses. It is therefore unclear how results from different assays compare, and what factors most significantly influence assay outcome. One such difference is that some laboratorie...
Main Authors: | Steven G Smith, Simone A Joosten, Virginie Verscheure, Ansar A Pathan, Helen McShane, Tom H M Ottenhoff, Hazel M Dockrell, Françoise Mascart |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2009-11-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC2776358?pdf=render |
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