Generation of mutation hotspots in ageing bacterial colonies

Generation of mutation hotspots in ageing bacterial colonies

Abstract How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adapt...

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Main Authors: Agnieszka Sekowska, Sofie Wendel, Emil C. Fischer, Morten H. H. Nørholm, Antoine Danchin
Format: Article
Language:English
Published: Nature Publishing Group 2016-12-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-016-0005-4
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spelling doaj-b564163529c64e73a48024b0ac5d51d42020-12-08T03:20:38ZengNature Publishing GroupScientific Reports2045-23222016-12-01611710.1038/s41598-016-0005-4Generation of mutation hotspots in ageing bacterial coloniesAgnieszka Sekowska0Sofie Wendel1Emil C. Fischer2Morten H. H. Nørholm3Antoine Danchin4Institute of Cardiometabolism and Nutrition, CHU Pitié-Salpêtrière, 47, Boulevard de l’HôpitalNovo Nordisk Foundation Center for Biosustainability, Technical University of DenmarkNovo Nordisk Foundation Center for Biosustainability, Technical University of DenmarkNovo Nordisk Foundation Center for Biosustainability, Technical University of DenmarkInstitute of Cardiometabolism and Nutrition, CHU Pitié-Salpêtrière, 47, Boulevard de l’HôpitalAbstract How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome, and over time, mutations increasingly were consistent with the involvement of 8-oxo-guanosine, formed exclusively on the transcribed strand. This work provides strong support for retromutagenesis as a general process creating adaptive mutations during ageing.https://doi.org/10.1038/s41598-016-0005-4
collection DOAJ
language English
format Article
sources DOAJ
author Agnieszka Sekowska
Sofie Wendel
Emil C. Fischer
Morten H. H. Nørholm
Antoine Danchin
spellingShingle Agnieszka Sekowska
Sofie Wendel
Emil C. Fischer
Morten H. H. Nørholm
Antoine Danchin
Generation of mutation hotspots in ageing bacterial colonies
Scientific Reports
author_facet Agnieszka Sekowska
Sofie Wendel
Emil C. Fischer
Morten H. H. Nørholm
Antoine Danchin
author_sort Agnieszka Sekowska
title Generation of mutation hotspots in ageing bacterial colonies
title_short Generation of mutation hotspots in ageing bacterial colonies
title_full Generation of mutation hotspots in ageing bacterial colonies
title_fullStr Generation of mutation hotspots in ageing bacterial colonies
title_full_unstemmed Generation of mutation hotspots in ageing bacterial colonies
title_sort generation of mutation hotspots in ageing bacterial colonies
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2016-12-01
description Abstract How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome, and over time, mutations increasingly were consistent with the involvement of 8-oxo-guanosine, formed exclusively on the transcribed strand. This work provides strong support for retromutagenesis as a general process creating adaptive mutations during ageing.
url https://doi.org/10.1038/s41598-016-0005-4
work_keys_str_mv AT agnieszkasekowska generationofmutationhotspotsinageingbacterialcolonies
AT sofiewendel generationofmutationhotspotsinageingbacterialcolonies
AT emilcfischer generationofmutationhotspotsinageingbacterialcolonies
AT mortenhhnørholm generationofmutationhotspotsinageingbacterialcolonies
AT antoinedanchin generationofmutationhotspotsinageingbacterialcolonies
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