A rapid ceramide synthase activity using NBD-sphinganine and solid phase extraction

A rapid ceramide synthase activity using NBD-sphinganine and solid phase extraction

Ceramides are synthesized by six mammalian ceramide synthases (CerSs), each of which uses fatty acyl-CoAs of different chain lengths for N-acylation of the sphingoid long-chain base. We now describe a rapid and reliable CerS assay that uses a fluorescent N-[6-[(7-nitrobenzo-2-oxa-1,3-diazol-4-yl) (N...

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Bibliographic Details
Main Authors: Rotem Tidhar, Kacee Sims, Eden Rosenfeld-Gur, Walter Shaw, Anthony H. Futerman
Format: Article
Language:English
Published: Elsevier 2015-01-01
Series:Journal of Lipid Research
Subjects:
ceramides
sphingolipids
in vitro assay
chromatography
enzymology
long-chain base
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520356522

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http://www.sciencedirect.com/science/article/pii/S0022227520356522

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