Denaturing cross-linking immunoprecipitation to identify footprints for RNA-binding proteins

Denaturing cross-linking immunoprecipitation to identify footprints for RNA-binding proteins

Summary: The isolation of protein-RNA complexes in the “denaturing cross-linked RNA immunoprecipitation” (dCLIP) protocol is based on biotin-tagging proteins of interest, UV cross-linking RNA to protein in vivo, RNase protection assay, and isolating RNA-protein complexes under denaturing conditions...

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Bibliographic Details
Main Authors: Michael Rosenberg, Vered Levy, Verena K. Maier, Barry Kesner, Roy Blum, Jeannie T. Lee
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Genetics
Genomics
Molecular Biology
Molecular/Chemical Probes
Protein Biochemistry
Systems biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721005256

Internet

http://www.sciencedirect.com/science/article/pii/S2666166721005256

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