Heritable Genomic Fragment Deletions and Small Indels in the Putative ENGase Gene Induced by CRISPR/Cas9 in Barley

Heritable Genomic Fragment Deletions and Small Indels in the Putative ENGase Gene Induced by CRISPR/Cas9 in Barley

Targeted genome editing with the CRISPR/Cas9 system has been used extensively for the selective mutation of plant genes. Here we used CRISPR/Cas9 to disrupt the putative barley (Hordeum vulgare cv. “Golden Promise”) endo-N-acetyl-β-D-glucosaminidase (ENGase) gene. Five single guide RNAs (sgRNAs) wer...

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Bibliographic Details
Main Authors: Eva Stoger, Eszter Kapusi, Maria Corcuera-Gómez, Stanislav Melnik
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-04-01
Series:Frontiers in Plant Science
Subjects:
barley
Hordeum vulgare
genome editing
crop
cereals
induced mutation
Online Access:http://journal.frontiersin.org/article/10.3389/fpls.2017.00540/full

Internet

http://journal.frontiersin.org/article/10.3389/fpls.2017.00540/full

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