Gateway-Compatible CRISPR-Cas9 Vectors and a Rapid Detection by High-Resolution Melting Curve Analysis

Gateway-Compatible CRISPR-Cas9 Vectors and a Rapid Detection by High-Resolution Melting Curve Analysis

CRISPR-Cas9 system rapidly became an indispensable tool in plant biology to perform targeted mutagenesis. A CRISPR-Cas9-mediated double strand break followed by non-homologous end joining (NHEJ) repair most frequently results in a single base pair deletion or insertions (indels), which is hard to de...

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Bibliographic Details
Main Authors: Cynthia J. Denbow, Samantha Lapins, Nick Dietz, Raelynn Scherer, Zachary L. Nimchuk, Sakiko Okumoto
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-07-01
Series:Frontiers in Plant Science
Subjects:
CRISPR/Cas9
high-resolution melting curve analysis
gene editing
indel detection
genome editing
Online Access:http://journal.frontiersin.org/article/10.3389/fpls.2017.01171/full

Internet

http://journal.frontiersin.org/article/10.3389/fpls.2017.01171/full

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