A Chronic Ocular-Hypertensive Rat Model induced by Injection of the Sclerosant Agent Polidocanol in the Aqueous Humor Outflow Pathway
Background: To induce a moderate chronic ocular hypertension (OHT) by injecting polidocanol, a foamed sclerosant drug, in the aqueous humor outflow pathway. Methods: Intraocular pressure (IOP) was monitored for up to 6 months. Pattern and full-field electroretinogram (PERG and ERG) were recorded and...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2019-06-01
|
Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/1422-0067/20/13/3209 |
id |
doaj-b6a2cce1639d447aaec99e53512f3975 |
---|---|
record_format |
Article |
spelling |
doaj-b6a2cce1639d447aaec99e53512f39752020-11-24T21:27:38ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-06-012013320910.3390/ijms20133209ijms20133209A Chronic Ocular-Hypertensive Rat Model induced by Injection of the Sclerosant Agent Polidocanol in the Aqueous Humor Outflow PathwayRomán Blanco0Gema Martinez-Navarrete1Consuelo Pérez-Rico2Francisco J. Valiente-Soriano3Marcelino Avilés-Trigueros4Javier Vicente5Eduardo Fernandez6Manuel Vidal-Sanz7Pedro de la Villa8Department of Surgery, Medical and Social Sciences, University of Alcalá, Alcalá de Henares, 28805 Madrid, SpainInstitute of Bioengineering, Miguel Hernandez University, Elche, 03202 Alicante, SpainDepartment of Surgery, Medical and Social Sciences, University of Alcalá, Alcalá de Henares, 28805 Madrid, SpainDepartment of Ophthalmology, University of Murcia and Institute Murciano of Investigation Biosanitaria-University Hospital Virgen de la Arrixaca (IMIB-Arrixaca), 30120 Murcia, SpainDepartment of Ophthalmology, University of Murcia and Institute Murciano of Investigation Biosanitaria-University Hospital Virgen de la Arrixaca (IMIB-Arrixaca), 30120 Murcia, SpainUniversidad Europea de Madrid, Villaviciosa de Odón, 28670 Madrid, SpainInstitute of Bioengineering, Miguel Hernandez University, Elche, 03202 Alicante, SpainDepartment of Ophthalmology, University of Murcia and Institute Murciano of Investigation Biosanitaria-University Hospital Virgen de la Arrixaca (IMIB-Arrixaca), 30120 Murcia, SpainInstituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), 28034 Madrid, SpainBackground: To induce a moderate chronic ocular hypertension (OHT) by injecting polidocanol, a foamed sclerosant drug, in the aqueous humor outflow pathway. Methods: Intraocular pressure (IOP) was monitored for up to 6 months. Pattern and full-field electroretinogram (PERG and ERG) were recorded and retinal ganglion cells (RGC) and retinal nerve fiber layer (RNFL) thickness were assessed in vivo with optical coherence tomography (OCT) and ex vivo using Brn3a immunohistochemistry. Results: In the first 3 weeks post-injection, a significant IOP elevation was observed in the treated eyes (18.47 ± 3.36 mmHg) when compared with the control fellow eyes (12.52 ± 2.84 mmHg) (<i>p</i> < 0.05). At 8 weeks, 65% (11/17) of intervention eyes had developed an IOP increase >25% over the baseline. PERG responses were seen to be significantly reduced in the hypertensive eyes (2.25 ± 0.24 µV) compared to control eyes (1.44 ± 0.19 µV) (<i>p</i> < 0.01) at week 3, whereas the ERG components (photoreceptor a-wave and bipolar cell b-wave) remained unaltered. By week 24, RNFL thinning and cell loss in the ganglion cell layer was first detected (2/13, 15.3%) as assessed by OCT and light microscopy. Conclusions: This novel OHT rat model, with moderate levels of chronically elevated IOP, and abnormal PERG shows selective functional impairment of RGC.https://www.mdpi.com/1422-0067/20/13/3209chronic ocular hypertensionoptical coherence tomographypattern and full-field electroretinogrampolidocanol injectionrat glaucoma model |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Román Blanco Gema Martinez-Navarrete Consuelo Pérez-Rico Francisco J. Valiente-Soriano Marcelino Avilés-Trigueros Javier Vicente Eduardo Fernandez Manuel Vidal-Sanz Pedro de la Villa |
spellingShingle |
Román Blanco Gema Martinez-Navarrete Consuelo Pérez-Rico Francisco J. Valiente-Soriano Marcelino Avilés-Trigueros Javier Vicente Eduardo Fernandez Manuel Vidal-Sanz Pedro de la Villa A Chronic Ocular-Hypertensive Rat Model induced by Injection of the Sclerosant Agent Polidocanol in the Aqueous Humor Outflow Pathway International Journal of Molecular Sciences chronic ocular hypertension optical coherence tomography pattern and full-field electroretinogram polidocanol injection rat glaucoma model |
author_facet |
Román Blanco Gema Martinez-Navarrete Consuelo Pérez-Rico Francisco J. Valiente-Soriano Marcelino Avilés-Trigueros Javier Vicente Eduardo Fernandez Manuel Vidal-Sanz Pedro de la Villa |
author_sort |
Román Blanco |
title |
A Chronic Ocular-Hypertensive Rat Model induced by Injection of the Sclerosant Agent Polidocanol in the Aqueous Humor Outflow Pathway |
title_short |
A Chronic Ocular-Hypertensive Rat Model induced by Injection of the Sclerosant Agent Polidocanol in the Aqueous Humor Outflow Pathway |
title_full |
A Chronic Ocular-Hypertensive Rat Model induced by Injection of the Sclerosant Agent Polidocanol in the Aqueous Humor Outflow Pathway |
title_fullStr |
A Chronic Ocular-Hypertensive Rat Model induced by Injection of the Sclerosant Agent Polidocanol in the Aqueous Humor Outflow Pathway |
title_full_unstemmed |
A Chronic Ocular-Hypertensive Rat Model induced by Injection of the Sclerosant Agent Polidocanol in the Aqueous Humor Outflow Pathway |
title_sort |
chronic ocular-hypertensive rat model induced by injection of the sclerosant agent polidocanol in the aqueous humor outflow pathway |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2019-06-01 |
description |
Background: To induce a moderate chronic ocular hypertension (OHT) by injecting polidocanol, a foamed sclerosant drug, in the aqueous humor outflow pathway. Methods: Intraocular pressure (IOP) was monitored for up to 6 months. Pattern and full-field electroretinogram (PERG and ERG) were recorded and retinal ganglion cells (RGC) and retinal nerve fiber layer (RNFL) thickness were assessed in vivo with optical coherence tomography (OCT) and ex vivo using Brn3a immunohistochemistry. Results: In the first 3 weeks post-injection, a significant IOP elevation was observed in the treated eyes (18.47 ± 3.36 mmHg) when compared with the control fellow eyes (12.52 ± 2.84 mmHg) (<i>p</i> < 0.05). At 8 weeks, 65% (11/17) of intervention eyes had developed an IOP increase >25% over the baseline. PERG responses were seen to be significantly reduced in the hypertensive eyes (2.25 ± 0.24 µV) compared to control eyes (1.44 ± 0.19 µV) (<i>p</i> < 0.01) at week 3, whereas the ERG components (photoreceptor a-wave and bipolar cell b-wave) remained unaltered. By week 24, RNFL thinning and cell loss in the ganglion cell layer was first detected (2/13, 15.3%) as assessed by OCT and light microscopy. Conclusions: This novel OHT rat model, with moderate levels of chronically elevated IOP, and abnormal PERG shows selective functional impairment of RGC. |
topic |
chronic ocular hypertension optical coherence tomography pattern and full-field electroretinogram polidocanol injection rat glaucoma model |
url |
https://www.mdpi.com/1422-0067/20/13/3209 |
work_keys_str_mv |
AT romanblanco achronicocularhypertensiveratmodelinducedbyinjectionofthesclerosantagentpolidocanolintheaqueoushumoroutflowpathway AT gemamartineznavarrete achronicocularhypertensiveratmodelinducedbyinjectionofthesclerosantagentpolidocanolintheaqueoushumoroutflowpathway AT consueloperezrico achronicocularhypertensiveratmodelinducedbyinjectionofthesclerosantagentpolidocanolintheaqueoushumoroutflowpathway AT franciscojvalientesoriano achronicocularhypertensiveratmodelinducedbyinjectionofthesclerosantagentpolidocanolintheaqueoushumoroutflowpathway AT marcelinoavilestrigueros achronicocularhypertensiveratmodelinducedbyinjectionofthesclerosantagentpolidocanolintheaqueoushumoroutflowpathway AT javiervicente achronicocularhypertensiveratmodelinducedbyinjectionofthesclerosantagentpolidocanolintheaqueoushumoroutflowpathway AT eduardofernandez achronicocularhypertensiveratmodelinducedbyinjectionofthesclerosantagentpolidocanolintheaqueoushumoroutflowpathway AT manuelvidalsanz achronicocularhypertensiveratmodelinducedbyinjectionofthesclerosantagentpolidocanolintheaqueoushumoroutflowpathway AT pedrodelavilla achronicocularhypertensiveratmodelinducedbyinjectionofthesclerosantagentpolidocanolintheaqueoushumoroutflowpathway AT romanblanco chronicocularhypertensiveratmodelinducedbyinjectionofthesclerosantagentpolidocanolintheaqueoushumoroutflowpathway AT gemamartineznavarrete chronicocularhypertensiveratmodelinducedbyinjectionofthesclerosantagentpolidocanolintheaqueoushumoroutflowpathway AT consueloperezrico chronicocularhypertensiveratmodelinducedbyinjectionofthesclerosantagentpolidocanolintheaqueoushumoroutflowpathway AT franciscojvalientesoriano chronicocularhypertensiveratmodelinducedbyinjectionofthesclerosantagentpolidocanolintheaqueoushumoroutflowpathway AT marcelinoavilestrigueros chronicocularhypertensiveratmodelinducedbyinjectionofthesclerosantagentpolidocanolintheaqueoushumoroutflowpathway AT javiervicente chronicocularhypertensiveratmodelinducedbyinjectionofthesclerosantagentpolidocanolintheaqueoushumoroutflowpathway AT eduardofernandez chronicocularhypertensiveratmodelinducedbyinjectionofthesclerosantagentpolidocanolintheaqueoushumoroutflowpathway AT manuelvidalsanz chronicocularhypertensiveratmodelinducedbyinjectionofthesclerosantagentpolidocanolintheaqueoushumoroutflowpathway AT pedrodelavilla chronicocularhypertensiveratmodelinducedbyinjectionofthesclerosantagentpolidocanolintheaqueoushumoroutflowpathway |
_version_ |
1725974255884042240 |