Nanoparticle Delivery of CRISPR/Cas9 for Genome Editing
The emerging clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated system (Cas) gene-editing system represents a promising tool for genome manipulation. However, its low intracellular delivery efficiency severely compromises its use and potency for clinical applications...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2021-05-01
|
Series: | Frontiers in Genetics |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2021.673286/full |
id |
doaj-b6ce71743dc84bde9f8e5f57cee7922c |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Li Duan Li Duan Li Duan Kan Ouyang Xiao Xu Xiao Xu Limei Xu Limei Xu Caining Wen Caining Wen Xiaoying Zhou Xiaoying Zhou Zhuan Qin Zhuan Qin Zhiyi Xu Zhiyi Xu Wei Sun Yujie Liang |
spellingShingle |
Li Duan Li Duan Li Duan Kan Ouyang Xiao Xu Xiao Xu Limei Xu Limei Xu Caining Wen Caining Wen Xiaoying Zhou Xiaoying Zhou Zhuan Qin Zhuan Qin Zhiyi Xu Zhiyi Xu Wei Sun Yujie Liang Nanoparticle Delivery of CRISPR/Cas9 for Genome Editing Frontiers in Genetics nanocarriers CRISPR/Cas9 delivery exosome modification |
author_facet |
Li Duan Li Duan Li Duan Kan Ouyang Xiao Xu Xiao Xu Limei Xu Limei Xu Caining Wen Caining Wen Xiaoying Zhou Xiaoying Zhou Zhuan Qin Zhuan Qin Zhiyi Xu Zhiyi Xu Wei Sun Yujie Liang |
author_sort |
Li Duan |
title |
Nanoparticle Delivery of CRISPR/Cas9 for Genome Editing |
title_short |
Nanoparticle Delivery of CRISPR/Cas9 for Genome Editing |
title_full |
Nanoparticle Delivery of CRISPR/Cas9 for Genome Editing |
title_fullStr |
Nanoparticle Delivery of CRISPR/Cas9 for Genome Editing |
title_full_unstemmed |
Nanoparticle Delivery of CRISPR/Cas9 for Genome Editing |
title_sort |
nanoparticle delivery of crispr/cas9 for genome editing |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Genetics |
issn |
1664-8021 |
publishDate |
2021-05-01 |
description |
The emerging clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated system (Cas) gene-editing system represents a promising tool for genome manipulation. However, its low intracellular delivery efficiency severely compromises its use and potency for clinical applications. Nanocarriers, such as liposomes, polymers, and inorganic nanoparticles, have shown great potential for gene delivery. The remarkable development of nanoparticles as non-viral carriers for the delivery of the CRISPR/Cas9 system has shown great promise for therapeutic applications. In this review, we briefly summarize the delivery components of the CRISPR/Cas9 system and report on the progress of nano-system development for CRISPR/Cas9 delivery. We also compare the advantages of various nano-delivery systems and their applications to deliver CRISPR/Cas9 for disease treatment. Nano-delivery systems can be modified to fulfill the tasks of targeting cells or tissues. We primarily emphasize the novel exosome-based CRISPR/Cas9 delivery system. Overall, we review the challenges, development trends, and application prospects of nanoparticle-based technology for CRISPR/Cas9 delivery. |
topic |
nanocarriers CRISPR/Cas9 delivery exosome modification |
url |
https://www.frontiersin.org/articles/10.3389/fgene.2021.673286/full |
work_keys_str_mv |
AT liduan nanoparticledeliveryofcrisprcas9forgenomeediting AT liduan nanoparticledeliveryofcrisprcas9forgenomeediting AT liduan nanoparticledeliveryofcrisprcas9forgenomeediting AT kanouyang nanoparticledeliveryofcrisprcas9forgenomeediting AT xiaoxu nanoparticledeliveryofcrisprcas9forgenomeediting AT xiaoxu nanoparticledeliveryofcrisprcas9forgenomeediting AT limeixu nanoparticledeliveryofcrisprcas9forgenomeediting AT limeixu nanoparticledeliveryofcrisprcas9forgenomeediting AT cainingwen nanoparticledeliveryofcrisprcas9forgenomeediting AT cainingwen nanoparticledeliveryofcrisprcas9forgenomeediting AT xiaoyingzhou nanoparticledeliveryofcrisprcas9forgenomeediting AT xiaoyingzhou nanoparticledeliveryofcrisprcas9forgenomeediting AT zhuanqin nanoparticledeliveryofcrisprcas9forgenomeediting AT zhuanqin nanoparticledeliveryofcrisprcas9forgenomeediting AT zhiyixu nanoparticledeliveryofcrisprcas9forgenomeediting AT zhiyixu nanoparticledeliveryofcrisprcas9forgenomeediting AT weisun nanoparticledeliveryofcrisprcas9forgenomeediting AT yujieliang nanoparticledeliveryofcrisprcas9forgenomeediting |
_version_ |
1721443309546635264 |
spelling |
doaj-b6ce71743dc84bde9f8e5f57cee7922c2021-05-12T07:20:37ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-05-011210.3389/fgene.2021.673286673286Nanoparticle Delivery of CRISPR/Cas9 for Genome EditingLi Duan0Li Duan1Li Duan2Kan Ouyang3Xiao Xu4Xiao Xu5Limei Xu6Limei Xu7Caining Wen8Caining Wen9Xiaoying Zhou10Xiaoying Zhou11Zhuan Qin12Zhuan Qin13Zhiyi Xu14Zhiyi Xu15Wei Sun16Yujie Liang17Department of Orthopedics, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaShenzhen Institute of Geriatrics, Shenzhen, ChinaGuangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen Second People’s Hospital, Shenzhen, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaGuangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen Second People’s Hospital, Shenzhen, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaGuangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen Second People’s Hospital, Shenzhen, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaGuangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen Second People’s Hospital, Shenzhen, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaGuangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen Second People’s Hospital, Shenzhen, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaGuangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen Second People’s Hospital, Shenzhen, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaGuangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen Second People’s Hospital, Shenzhen, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaDepartment of Child and Adolescent Psychiatry, Shenzhen Kangning Hospital, Shenzhen Mental Health Center, Shenzhen Key Laboratory for Psychological Healthcare & Shenzhen Institute of Mental Health, Shenzhen, ChinaThe emerging clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated system (Cas) gene-editing system represents a promising tool for genome manipulation. However, its low intracellular delivery efficiency severely compromises its use and potency for clinical applications. Nanocarriers, such as liposomes, polymers, and inorganic nanoparticles, have shown great potential for gene delivery. The remarkable development of nanoparticles as non-viral carriers for the delivery of the CRISPR/Cas9 system has shown great promise for therapeutic applications. In this review, we briefly summarize the delivery components of the CRISPR/Cas9 system and report on the progress of nano-system development for CRISPR/Cas9 delivery. We also compare the advantages of various nano-delivery systems and their applications to deliver CRISPR/Cas9 for disease treatment. Nano-delivery systems can be modified to fulfill the tasks of targeting cells or tissues. We primarily emphasize the novel exosome-based CRISPR/Cas9 delivery system. Overall, we review the challenges, development trends, and application prospects of nanoparticle-based technology for CRISPR/Cas9 delivery.https://www.frontiersin.org/articles/10.3389/fgene.2021.673286/fullnanocarriersCRISPR/Cas9deliveryexosomemodification |