PrimerSeq: Design and Visualization of RT-PCR Primers for Alternative Splicing Using RNA-seq Data

PrimerSeq: Design and Visualization of RT-PCR Primers for Alternative Splicing Using RNA-seq Data

The vast majority of multi-exon genes in higher eukaryotes are alternatively spliced and changes in alternative splicing (AS) can impact gene function or cause disease. High-throughput RNA sequencing (RNA-seq) has become a powerful technology for transcriptome-wide analysis of AS, but RT-PCR still r...

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Bibliographic Details
Main Authors: Collin Tokheim, Juw Won Park, Yi Xing
Format: Article
Language:English
Published: Elsevier 2014-04-01
Series:Genomics, Proteomics & Bioinformatics
Subjects:
Alternative splicing
RNA-seq
Primer design
Transcriptome
Visualization
Online Access:http://www.sciencedirect.com/science/article/pii/S1672022914000254

Internet

http://www.sciencedirect.com/science/article/pii/S1672022914000254

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