A Quantitative Arabidopsis IRE1a Ribonuclease-Dependent in vitro mRNA Cleavage Assay for Functional Studies of Substrate Splicing and Decay Activities

A Quantitative Arabidopsis IRE1a Ribonuclease-Dependent in vitro mRNA Cleavage Assay for Functional Studies of Substrate Splicing and Decay Activities

The unfolded protein response (UPR) is an adaptive eukaryotic reaction that controls the protein folding capacities of the endoplasmic reticulum (ER). The most ancient and well-conserved component of the UPR is Inositol-Requiring Enzyme 1 (IRE1). Arabidopsis IRE1a (AtIRE1) is a transmembrane sensor...

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Bibliographic Details
Main Authors: Danish Diwan, Xiaoyu Liu, Caroline F. Andrews, Karolina M. Pajerowska-Mukhtar
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-07-01
Series:Frontiers in Plant Science
Subjects:
Arabidopsis thaliana
Nicotiana benthamiana
unfolded protein response
IRE1
bZIP60
in vitro mRNA cleavage
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2021.707378/full

Internet

https://www.frontiersin.org/articles/10.3389/fpls.2021.707378/full

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