3-Bromo-4,5-dihydroxybenzaldehyde Enhances the Level of Reduced Glutathione via the Nrf2-Mediated Pathway in Human Keratinocytes

3-Bromo-4,5-dihydroxybenzaldehyde Enhances the Level of Reduced Glutathione via the Nrf2-Mediated Pathway in Human Keratinocytes

A natural bromophenol found in seaweeds, 3-bromo-4,5-dihydroxybenzaldehyde (BDB), has been shown to possess antioxidant effects. This study aimed to investigate the mechanism by which BDB protects skin cells subjected to oxidative stress. The effect of BDB on the protein and mRNA levels of glutathio...

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Main Authors: Ki Cheon Kim, Yu Jae Hyun, Susara Ruwan Kumara Madduma Hewage, Mei Jing Piao, Kyoung Ah Kang, Hee Kyoung Kang, Young Sang Koh, Mee Jung Ahn, Jin Won Hyun
Format: Article
Language:English
Published: MDPI AG 2017-09-01
Series:Marine Drugs
Subjects:
3-bromo-4,5-dihydroxybenzaldehyde
glutathione
oxidative stress
glutathione synthetase
NF-E2 related factor 2
Online Access:https://www.mdpi.com/1660-3397/15/9/291
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spelling doaj-b75187cdef514cbb84a05bac9833f1fb2020-11-25T00:49:50ZengMDPI AGMarine Drugs1660-33972017-09-0115929110.3390/md15090291md150902913-Bromo-4,5-dihydroxybenzaldehyde Enhances the Level of Reduced Glutathione via the Nrf2-Mediated Pathway in Human KeratinocytesKi Cheon Kim0Yu Jae Hyun1Susara Ruwan Kumara Madduma Hewage2Mei Jing Piao3Kyoung Ah Kang4Hee Kyoung Kang5Young Sang Koh6Mee Jung Ahn7Jin Won Hyun8Jeju National University School of Medicine and Jeju Research Center for Natural Medicine, Jeju 63243, KoreaJeju National University School of Medicine and Jeju Research Center for Natural Medicine, Jeju 63243, KoreaJeju National University School of Medicine and Jeju Research Center for Natural Medicine, Jeju 63243, KoreaJeju National University School of Medicine and Jeju Research Center for Natural Medicine, Jeju 63243, KoreaJeju National University School of Medicine and Jeju Research Center for Natural Medicine, Jeju 63243, KoreaJeju National University School of Medicine and Jeju Research Center for Natural Medicine, Jeju 63243, KoreaJeju National University School of Medicine and Jeju Research Center for Natural Medicine, Jeju 63243, KoreaLaboratory of Veterinary Anatomy, College of Veterinary Medicine, Jeju National University, Jeju 63243, KoreaJeju National University School of Medicine and Jeju Research Center for Natural Medicine, Jeju 63243, KoreaA natural bromophenol found in seaweeds, 3-bromo-4,5-dihydroxybenzaldehyde (BDB), has been shown to possess antioxidant effects. This study aimed to investigate the mechanism by which BDB protects skin cells subjected to oxidative stress. The effect of BDB on the protein and mRNA levels of glutathione-related enzymes and the cell survival of human keratinocytes (HaCaT cells) was investigated. BDB treatment increased the protein and mRNA levels of glutathione synthesizing enzymes and enhanced the production of reduced glutathione in HaCaT cells. Furthermore, BDB activated NF-E2-related factor 2 (Nrf2) and promoted its localization into the nucleus by phosphorylating its up-stream signaling proteins, extracellular signal–regulated kinase and protein kinase B. Thus, BDB increased the production of reduced glutathione and established cellular protection against oxidative stress via an Nrf2-mediated pathway.https://www.mdpi.com/1660-3397/15/9/2913-bromo-4,5-dihydroxybenzaldehydeglutathioneoxidative stressglutathione synthetaseNF-E2 related factor 2
collection DOAJ
language English
format Article
sources DOAJ
author Ki Cheon Kim
Yu Jae Hyun
Susara Ruwan Kumara Madduma Hewage
Mei Jing Piao
Kyoung Ah Kang
Hee Kyoung Kang
Young Sang Koh
Mee Jung Ahn
Jin Won Hyun
spellingShingle Ki Cheon Kim
Yu Jae Hyun
Susara Ruwan Kumara Madduma Hewage
Mei Jing Piao
Kyoung Ah Kang
Hee Kyoung Kang
Young Sang Koh
Mee Jung Ahn
Jin Won Hyun
3-Bromo-4,5-dihydroxybenzaldehyde Enhances the Level of Reduced Glutathione via the Nrf2-Mediated Pathway in Human Keratinocytes
Marine Drugs
3-bromo-4,5-dihydroxybenzaldehyde
glutathione
oxidative stress
glutathione synthetase
NF-E2 related factor 2
author_facet Ki Cheon Kim
Yu Jae Hyun
Susara Ruwan Kumara Madduma Hewage
Mei Jing Piao
Kyoung Ah Kang
Hee Kyoung Kang
Young Sang Koh
Mee Jung Ahn
Jin Won Hyun
author_sort Ki Cheon Kim
title 3-Bromo-4,5-dihydroxybenzaldehyde Enhances the Level of Reduced Glutathione via the Nrf2-Mediated Pathway in Human Keratinocytes
title_short 3-Bromo-4,5-dihydroxybenzaldehyde Enhances the Level of Reduced Glutathione via the Nrf2-Mediated Pathway in Human Keratinocytes
title_full 3-Bromo-4,5-dihydroxybenzaldehyde Enhances the Level of Reduced Glutathione via the Nrf2-Mediated Pathway in Human Keratinocytes
title_fullStr 3-Bromo-4,5-dihydroxybenzaldehyde Enhances the Level of Reduced Glutathione via the Nrf2-Mediated Pathway in Human Keratinocytes
title_full_unstemmed 3-Bromo-4,5-dihydroxybenzaldehyde Enhances the Level of Reduced Glutathione via the Nrf2-Mediated Pathway in Human Keratinocytes
title_sort 3-bromo-4,5-dihydroxybenzaldehyde enhances the level of reduced glutathione via the nrf2-mediated pathway in human keratinocytes
publisher MDPI AG
series Marine Drugs
issn 1660-3397
publishDate 2017-09-01
description A natural bromophenol found in seaweeds, 3-bromo-4,5-dihydroxybenzaldehyde (BDB), has been shown to possess antioxidant effects. This study aimed to investigate the mechanism by which BDB protects skin cells subjected to oxidative stress. The effect of BDB on the protein and mRNA levels of glutathione-related enzymes and the cell survival of human keratinocytes (HaCaT cells) was investigated. BDB treatment increased the protein and mRNA levels of glutathione synthesizing enzymes and enhanced the production of reduced glutathione in HaCaT cells. Furthermore, BDB activated NF-E2-related factor 2 (Nrf2) and promoted its localization into the nucleus by phosphorylating its up-stream signaling proteins, extracellular signal–regulated kinase and protein kinase B. Thus, BDB increased the production of reduced glutathione and established cellular protection against oxidative stress via an Nrf2-mediated pathway.
topic 3-bromo-4,5-dihydroxybenzaldehyde
glutathione
oxidative stress
glutathione synthetase
NF-E2 related factor 2
url https://www.mdpi.com/1660-3397/15/9/291
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AT yujaehyun 3bromo45dihydroxybenzaldehydeenhancesthelevelofreducedglutathioneviathenrf2mediatedpathwayinhumankeratinocytes
AT susararuwankumaramaddumahewage 3bromo45dihydroxybenzaldehydeenhancesthelevelofreducedglutathioneviathenrf2mediatedpathwayinhumankeratinocytes
AT meijingpiao 3bromo45dihydroxybenzaldehydeenhancesthelevelofreducedglutathioneviathenrf2mediatedpathwayinhumankeratinocytes
AT kyoungahkang 3bromo45dihydroxybenzaldehydeenhancesthelevelofreducedglutathioneviathenrf2mediatedpathwayinhumankeratinocytes
AT heekyoungkang 3bromo45dihydroxybenzaldehydeenhancesthelevelofreducedglutathioneviathenrf2mediatedpathwayinhumankeratinocytes
AT youngsangkoh 3bromo45dihydroxybenzaldehydeenhancesthelevelofreducedglutathioneviathenrf2mediatedpathwayinhumankeratinocytes
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AT jinwonhyun 3bromo45dihydroxybenzaldehydeenhancesthelevelofreducedglutathioneviathenrf2mediatedpathwayinhumankeratinocytes
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