A Bacterial Biosensor for Oxidative Stress Using the Constitutively Expressed Redox-Sensitive Protein roGFP2

A Bacterial Biosensor for Oxidative Stress Using the Constitutively Expressed Redox-Sensitive Protein roGFP2

A highly specific, high throughput-amenable bacterial biosensor for chemically induced cellular oxidation was developed using constitutively expressed redox-sensitive green fluorescent protein roGFP2 in E. coli (E. coli-roGFP2). Disulfide formation between two key cysteine residues of roGFP2 was ass...

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Bibliographic Details
Main Authors: Carlos R. Arias-Barreiro, Keisuke Okazaki, Apostolos Koutsaftis, Salmaan H. Inayat-Hussain, Akio Tani, Maki Katsuhara, Kazuhide Kimbara, Izumi C. Mori
Format: Article
Language:English
Published: MDPI AG 2010-06-01
Series:Sensors
Subjects:
oxidative biosensor
redox-sensitive GFP
ratiometric measurement
ROS
environmental stressors
Online Access:http://www.mdpi.com/1424-8220/10/7/6290/

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http://www.mdpi.com/1424-8220/10/7/6290/

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