Acrylonitrile‐Mediated Nascent RNA Sequencing for Transcriptome‐Wide Profiling of Cellular RNA Dynamics

Acrylonitrile‐Mediated Nascent RNA Sequencing for Transcriptome‐Wide Profiling of Cellular RNA Dynamics

Abstract RNA sequencing has greatly facilitated gene expression studies but is weak in studying temporal RNA dynamics; this issue can be addressed by analyzing nascent RNAs. A famous method for nascent RNA analysis is metabolic labeling with noncanonic nucleoside followed by affinity purification, h...

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Bibliographic Details
Main Authors: Yuqi Chen, Fan Wu, Zonggui Chen, Zhiyong He, Qi Wei, Weiwu Zeng, Kun Chen, Feng Xiao, Yushu Yuan, Xiaocheng Weng, Yu Zhou, Xiang Zhou
Format: Article
Language:English
Published: Wiley 2020-04-01
Series:Advanced Science
Subjects:
acrylonitrile
G‐quadruplex
nascent RNA sequencing
RNA dynamics, transcriptome‐wide profiling
uridine‐to‐cytidine conversion
Online Access:https://doi.org/10.1002/advs.201900997

Internet

https://doi.org/10.1002/advs.201900997

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