Extraction Protocols for Individual Zebrafish's Ventricle Myosin and Skeletal Muscle Actin for In vitro Motility Assays

The in vitro motility assay (IVMA) is a technique that enables the measurement of the interaction between actin and myosin providing a relatively simple model to understand the mechanical muscle function. For actin-myosin IVMA, myosin is immobilized in a measurement chamber, where it converts chemic...

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Bibliographic Details
Main Authors: Lisa-Mareike Scheid, Cornelia Weber, Nasrin Bopp, Matias Mosqueira, Rainer H. A. Fink
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-05-01
Series:Frontiers in Physiology
Subjects:
Online Access:http://journal.frontiersin.org/article/10.3389/fphys.2017.00367/full