Optimus Primer: A PCR enrichment primer design program for next-generation sequencing of human exonic regions

<p>Abstract</p> <p>Background</p> <p>Polymerase chain reaction (PCR) remains a simple, flexible, and inexpensive method for enriching genomic regions of interest for next-generation sequencing. In order to utilize PCR in this context, a major challenge facing researcher...

Full description

Bibliographic Details
Main Authors: Rioux John D, Beaudoin Mélissa, Guelpa Paul, Lo Ken, Brown Andrew MK, Tardif Jean-Claude, Phillips Michael S, Lettre Guillaume
Format: Article
Language:English
Published: BMC 2010-07-01
Series:BMC Research Notes
Online Access:http://www.biomedcentral.com/1756-0500/3/185