Design of targeted primers based on 16S rRNA sequences in meta-transcriptomic datasets and identification of a novel taxonomic group in the Asgard archaea

Abstract Background Amplification of small subunit (SSU) rRNA genes with universal primers is a common method used to assess microbial populations in various environmental samples. However, owing to limitations in coverage of these universal primers, some microorganisms remain unidentified. The pres...

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Bibliographic Details
Main Authors: Ru-Yi Zhang, Bin Zou, Yong-Wei Yan, Che Ok Jeon, Meng Li, Mingwei Cai, Zhe-Xue Quan
Format: Article
Language:English
Published: BMC 2020-02-01
Series:BMC Microbiology
Subjects:
Online Access:https://doi.org/10.1186/s12866-020-1707-0