TLR4 Response to LPS Is Reinforced by Urokinase Receptor
GPI-anchored uPAR is the receptor for the extracellular serine protease urokinase-type plasminogen activator (uPA). Though uPAR role in inflammatory processes is documented, underlying mechanisms are not fully understood. In this study we demonstrate that uPAR is a part of Toll-like receptor 4 (TLR4...
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2020-12-01
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doaj-b81681ac123245f7b9d9565a8bb4d2b62020-12-09T13:16:34ZengFrontiers Media S.A.Frontiers in Immunology1664-32242020-12-011110.3389/fimmu.2020.573550573550TLR4 Response to LPS Is Reinforced by Urokinase ReceptorYulia Kiyan0Sergey Tkachuk1Song Rong2Anna Gorrasi3Pia Ragno4Inna Dumler5Hermann Haller6Nelli Shushakova7Nelli Shushakova8Nephrology Department, Hannover Medical School, Hannover, GermanyNephrology Department, Hannover Medical School, Hannover, GermanyPhenos GmbH, Hannover, GermanyNouscom, Rome, ItalyDepartment of Chemistry and Biology, University of Salerno, Salerno, ItalyNephrology Department, Hannover Medical School, Hannover, GermanyNephrology Department, Hannover Medical School, Hannover, GermanyNephrology Department, Hannover Medical School, Hannover, GermanyPhenos GmbH, Hannover, GermanyGPI-anchored uPAR is the receptor for the extracellular serine protease urokinase-type plasminogen activator (uPA). Though uPAR role in inflammatory processes is documented, underlying mechanisms are not fully understood. In this study we demonstrate that uPAR is a part of Toll-like receptor 4 (TLR4) interactome. Downregulation of uPAR expression resulted in diminished LPS-induced TLR4 signaling, less activation of NFκB, and decreased secretion of inflammatory mediators in myeloid and non-myeloid cells in vitro. In vivo uPAR−/− mice demonstrated better survival, strongly diminished inflammatory response and better organ functions in cecal ligation and puncture mouse polymicrobial sepsis model. Mechanistically, GPI-uPAR and soluble uPAR colocalized with TLR4 on the cell membrane and interacted with scavenger receptor CD36. Our data show that uPAR can interfere with innate immunity response via TLR4 and this mechanism represents a potentially important target in inflammation and sepsis therapy.https://www.frontiersin.org/articles/10.3389/fimmu.2020.573550/fullurokinase receptorCD36sepsisLPSTLR4 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yulia Kiyan Sergey Tkachuk Song Rong Anna Gorrasi Pia Ragno Inna Dumler Hermann Haller Nelli Shushakova Nelli Shushakova |
spellingShingle |
Yulia Kiyan Sergey Tkachuk Song Rong Anna Gorrasi Pia Ragno Inna Dumler Hermann Haller Nelli Shushakova Nelli Shushakova TLR4 Response to LPS Is Reinforced by Urokinase Receptor Frontiers in Immunology urokinase receptor CD36 sepsis LPS TLR4 |
author_facet |
Yulia Kiyan Sergey Tkachuk Song Rong Anna Gorrasi Pia Ragno Inna Dumler Hermann Haller Nelli Shushakova Nelli Shushakova |
author_sort |
Yulia Kiyan |
title |
TLR4 Response to LPS Is Reinforced by Urokinase Receptor |
title_short |
TLR4 Response to LPS Is Reinforced by Urokinase Receptor |
title_full |
TLR4 Response to LPS Is Reinforced by Urokinase Receptor |
title_fullStr |
TLR4 Response to LPS Is Reinforced by Urokinase Receptor |
title_full_unstemmed |
TLR4 Response to LPS Is Reinforced by Urokinase Receptor |
title_sort |
tlr4 response to lps is reinforced by urokinase receptor |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2020-12-01 |
description |
GPI-anchored uPAR is the receptor for the extracellular serine protease urokinase-type plasminogen activator (uPA). Though uPAR role in inflammatory processes is documented, underlying mechanisms are not fully understood. In this study we demonstrate that uPAR is a part of Toll-like receptor 4 (TLR4) interactome. Downregulation of uPAR expression resulted in diminished LPS-induced TLR4 signaling, less activation of NFκB, and decreased secretion of inflammatory mediators in myeloid and non-myeloid cells in vitro. In vivo uPAR−/− mice demonstrated better survival, strongly diminished inflammatory response and better organ functions in cecal ligation and puncture mouse polymicrobial sepsis model. Mechanistically, GPI-uPAR and soluble uPAR colocalized with TLR4 on the cell membrane and interacted with scavenger receptor CD36. Our data show that uPAR can interfere with innate immunity response via TLR4 and this mechanism represents a potentially important target in inflammation and sepsis therapy. |
topic |
urokinase receptor CD36 sepsis LPS TLR4 |
url |
https://www.frontiersin.org/articles/10.3389/fimmu.2020.573550/full |
work_keys_str_mv |
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