Serine Hydroxymethyltransferase from the Cold Adapted Microorganism Psychromonas ingrahamii: A Low Temperature Active Enzyme with Broad Substrate Specificity

Serine Hydroxymethyltransferase from the Cold Adapted Microorganism Psychromonas ingrahamii: A Low Temperature Active Enzyme with Broad Substrate Specificity

Serine hydroxymethyltransferase from the psychrophilic microorganism Psychromonas ingrahamii was expressed in Escherichia coli and purified as a His-tag fusion protein. The enzyme was characterized with respect to its spectroscopic, catalytic, and thermodynamic properties. The properties of the psyc...

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Bibliographic Details
Main Authors: Stefano Pascarella, Sebastiana Angelaccio, Rita Florio, Guido Festa, Valerio Consalvi
Format: Article
Language:English
Published: MDPI AG 2012-01-01
Series:International Journal of Molecular Sciences
Subjects:
cold adaptation
serine hydroxymethyltransferase
structural flexibility
temperature dependence of enzyme activity
catalytic promiscuity
pyridoxal-5′-phosphate
cofactor-mediated stabilization
psychrophiles
Psychromonas ingrahamii
Online Access:http://www.mdpi.com/1422-0067/13/2/1314/

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http://www.mdpi.com/1422-0067/13/2/1314/

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