Affinity chromatography of lipase with hydrophobic ligands coupled to cyanogen bromide-activated agarose
The behavior of lipase produced by Pseudomonas mephitica var. lipolytica toward hydrophobic residues coupled to spacer gels that were prepared by coupling a primary amine to CNBr-activated agarose, was studied. The lipase adsorbed on the ligand of a long unbranched aliphatic chain, a benzene ring, o...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
1976-07-01
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Series: | Journal of Lipid Research |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520349142 |