Affinity chromatography of lipase with hydrophobic ligands coupled to cyanogen bromide-activated agarose
The behavior of lipase produced by Pseudomonas mephitica var. lipolytica toward hydrophobic residues coupled to spacer gels that were prepared by coupling a primary amine to CNBr-activated agarose, was studied. The lipase adsorbed on the ligand of a long unbranched aliphatic chain, a benzene ring, o...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1976-07-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520349142 |