In Vitro Replication of Recombinant Marek’S Disease Viruses Constructed from Field Strains Lacking Meq and Vtr Oncogenes
The study describes construction of five recombinant very virulent (vv) and very virulent plus (vv+) strains lacking meq and viral telomerase (vTR). Deletion of both copies of meq and vTR was achieved by Red E/T recombination in GS1783 E. coli cells. The constructed bacterial artificial chromosome (...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
Sciendo
2013-06-01
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Series: | Bulletin of the Veterinary Institute in Pulawy |
Subjects: | |
Online Access: | https://doi.org/10.2478/bvip-2013-0027 |