Artifactual pyrosequencing reads in multiple-displacement-amplified sediment metagenomes from the Red Sea

The Multiple Displacement Amplification (MDA) protocol is reported to introduce different artifacts into DNA samples with impurities. In this study, we report an artifactual effect of MDA with sediment DNA samples from a deep-sea brine basin in the Red Sea. In the metagenomes, we showed the presence...

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Bibliographic Details
Main Authors: Yong Wang, On On Lee, Jiang Ke Yang, Tie Gang Li, Pei Yuan Qian
Format: Article
Language:English
Published: PeerJ Inc. 2013-04-01
Series:PeerJ
Subjects:
MDA
Online Access:https://peerj.com/articles/69.pdf