A Novel Rat Tail Needle Minimally Invasive Puncture Model Using Three-Dimensional Printing for Disk Degeneration and Progressive Osteogenesis Research
Many studies focused on the annulus fibrosus (AF) injury in rodent tail model for the intervertebral disk degeneration (IDD) research. However, previous studies caused tremendous injury of intervertebral disk (IVD) by penetrating whole disk. This study aimed to build a progressive IDD rodent tail mo...
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2021-06-01
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doaj-b9259d267f6846428cc0d633642de4a42021-06-03T05:44:49ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-06-01910.3389/fcell.2021.587399587399A Novel Rat Tail Needle Minimally Invasive Puncture Model Using Three-Dimensional Printing for Disk Degeneration and Progressive Osteogenesis ResearchDongdong Xia0Meijun Yan1Xin Yin2Wenhao Hu3Chi Zhang4Baiwen Hu5Ting Ge6Xiaochuan Wu7Jin Xiao8Liang Gao9Junqi Liu10Jun Tan11Department of Orthopedics, Ningbo First Hospital, Ningbo, ChinaDepartment of Orthopaedics, Shanghai General Hospital, Shanghai, ChinaDepartment of Orthopedics, Fourth Medical Center of PLA General Hospital, Beijing, ChinaDepartment of Orthopedics, Fourth Medical Center of PLA General Hospital, Beijing, ChinaDepartment of Orthopedics, Ningbo First Hospital, Ningbo, ChinaDepartment of Orthopedics, Ningbo First Hospital, Ningbo, ChinaDepartment of Orthopedics, Ningbo First Hospital, Ningbo, ChinaDepartment of Orthopedics, Ningbo First Hospital, Ningbo, ChinaDepartment of Orthopedics, Ningbo First Hospital, Ningbo, ChinaCenter for Clinical Medicine, Hua Tuo Institute of Medical Innovation (HTIMI), Wuhan, ChinaDepartment of Radiotherapy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Orthopedics, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, ChinaMany studies focused on the annulus fibrosus (AF) injury in rodent tail model for the intervertebral disk degeneration (IDD) research. However, previous studies caused tremendous injury of intervertebral disk (IVD) by penetrating whole disk. This study aimed to build a progressive IDD rodent tail model by a novel device for precise and minimally invasive puncture in AF. A precise puncture device was customized by 3D Printing Technique. 40 rodent tail IVDs were randomly grouped as follows: group A, non-puncture; group B, annulus needle puncture (ANP) for 4 week; group C, ANP for 8 week; and group D, ANP for 12 week. Pre- and post-puncture IVD height on radiographs and IVD signal intensity on T2 magnetic resonance imaging (MRI) were measured. Average bone density (ABD) on the end of coccygeal vertebrae between punctured disk was measured on the radiographs. Hematoxylin and eosin, TUNEL staining methods, immunofluorescence for cleaved-caspas3 and immunohistochemistry for aggrecan and collagen II were performed. Progressively and significantly increasing IVD height loss and degenerative grade were observed following the time points. The ABD was respectively, 81.20 ± 4.63 in group A, 83.93±3.18 in group B, 92.65 ± 4.32 in group C, 98.87 ± 6.69 in group D. In both group C and group D, there were significant differences with group A. In histology, increasing number of AF cells was noted in group B. In both group C and D, the fissures in AF were obviously observed, and a marked reduction of AF cells were also observed. In all ANP groups, there were significant decrease in number of NP cells, as well as aggrecan and collagen II contents. TUNEL assay showed cellular apoptosis were stimulated in all puncture group, especially in group D. A progressive IDD rat model could be standardly established by the micro-injury IVD puncture using a novel 3D printing device. This animal model provided a potential application for research of progressive hyperosteogeny following IDD development.https://www.frontiersin.org/articles/10.3389/fcell.2021.587399/fulldisk degenerationrodent tail modelvertebral hyperosteogenyminimally invasive procedure3D printing technique |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dongdong Xia Meijun Yan Xin Yin Wenhao Hu Chi Zhang Baiwen Hu Ting Ge Xiaochuan Wu Jin Xiao Liang Gao Junqi Liu Jun Tan |
spellingShingle |
Dongdong Xia Meijun Yan Xin Yin Wenhao Hu Chi Zhang Baiwen Hu Ting Ge Xiaochuan Wu Jin Xiao Liang Gao Junqi Liu Jun Tan A Novel Rat Tail Needle Minimally Invasive Puncture Model Using Three-Dimensional Printing for Disk Degeneration and Progressive Osteogenesis Research Frontiers in Cell and Developmental Biology disk degeneration rodent tail model vertebral hyperosteogeny minimally invasive procedure 3D printing technique |
author_facet |
Dongdong Xia Meijun Yan Xin Yin Wenhao Hu Chi Zhang Baiwen Hu Ting Ge Xiaochuan Wu Jin Xiao Liang Gao Junqi Liu Jun Tan |
author_sort |
Dongdong Xia |
title |
A Novel Rat Tail Needle Minimally Invasive Puncture Model Using Three-Dimensional Printing for Disk Degeneration and Progressive Osteogenesis Research |
title_short |
A Novel Rat Tail Needle Minimally Invasive Puncture Model Using Three-Dimensional Printing for Disk Degeneration and Progressive Osteogenesis Research |
title_full |
A Novel Rat Tail Needle Minimally Invasive Puncture Model Using Three-Dimensional Printing for Disk Degeneration and Progressive Osteogenesis Research |
title_fullStr |
A Novel Rat Tail Needle Minimally Invasive Puncture Model Using Three-Dimensional Printing for Disk Degeneration and Progressive Osteogenesis Research |
title_full_unstemmed |
A Novel Rat Tail Needle Minimally Invasive Puncture Model Using Three-Dimensional Printing for Disk Degeneration and Progressive Osteogenesis Research |
title_sort |
novel rat tail needle minimally invasive puncture model using three-dimensional printing for disk degeneration and progressive osteogenesis research |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cell and Developmental Biology |
issn |
2296-634X |
publishDate |
2021-06-01 |
description |
Many studies focused on the annulus fibrosus (AF) injury in rodent tail model for the intervertebral disk degeneration (IDD) research. However, previous studies caused tremendous injury of intervertebral disk (IVD) by penetrating whole disk. This study aimed to build a progressive IDD rodent tail model by a novel device for precise and minimally invasive puncture in AF. A precise puncture device was customized by 3D Printing Technique. 40 rodent tail IVDs were randomly grouped as follows: group A, non-puncture; group B, annulus needle puncture (ANP) for 4 week; group C, ANP for 8 week; and group D, ANP for 12 week. Pre- and post-puncture IVD height on radiographs and IVD signal intensity on T2 magnetic resonance imaging (MRI) were measured. Average bone density (ABD) on the end of coccygeal vertebrae between punctured disk was measured on the radiographs. Hematoxylin and eosin, TUNEL staining methods, immunofluorescence for cleaved-caspas3 and immunohistochemistry for aggrecan and collagen II were performed. Progressively and significantly increasing IVD height loss and degenerative grade were observed following the time points. The ABD was respectively, 81.20 ± 4.63 in group A, 83.93±3.18 in group B, 92.65 ± 4.32 in group C, 98.87 ± 6.69 in group D. In both group C and group D, there were significant differences with group A. In histology, increasing number of AF cells was noted in group B. In both group C and D, the fissures in AF were obviously observed, and a marked reduction of AF cells were also observed. In all ANP groups, there were significant decrease in number of NP cells, as well as aggrecan and collagen II contents. TUNEL assay showed cellular apoptosis were stimulated in all puncture group, especially in group D. A progressive IDD rat model could be standardly established by the micro-injury IVD puncture using a novel 3D printing device. This animal model provided a potential application for research of progressive hyperosteogeny following IDD development. |
topic |
disk degeneration rodent tail model vertebral hyperosteogeny minimally invasive procedure 3D printing technique |
url |
https://www.frontiersin.org/articles/10.3389/fcell.2021.587399/full |
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